| 1株苯并［a］芘高效降解菌的筛选与降解特性 |
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| 引用本文： | 蔡瀚,尹华,叶锦韶,常晶晶,彭辉,张娜,何宝燕.1株苯并［a］芘高效降解菌的筛选与降解特性[J].环境科学,2013,34(5):1937-1944. |
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| 作者姓名： | 蔡瀚 尹华 叶锦韶 常晶晶 彭辉 张娜 何宝燕 |
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| 作者单位： | 1. 暨南大学环境工程系,广东省高校毒害性污染物防治与生物修复重点实验室,广州510632
2. 华南理工大学环境科学与工程学院,广州510006;工业聚集区污染控制与生态修复教育部重点实验室,广州510006 |
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| 基金项目： | 国家自然科学基金委-广东联合基金重点项目(U0933002); 国家自然科学基金项目(50978122); 广州市科技计划项目(2011J4300075); 暨南大学科研培育与创新基金项目(11612448) |
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| 摘 要： | 从广东省贵屿镇受污染河涌底泥中筛选出1株能利用苯并a]芘(BaP)作为唯一碳源和能源生长的高效降解菌株.经生理生化试验和16S rRNA序列测定,鉴定该菌为短短芽胞杆菌(Brevibacillus brevis).该菌在7 d内对1 mg.L-1BaP的降解率达51.35%.降解性能研究表明,pH、温度、投菌量、BaP初始浓度和处理时间等是影响BaP降解的重要因素.B.brevis对pH与温度均有较广泛的耐受范围,在pH 2～12和温度为25～40℃的范围内对BaP均有降解能力,中性(pH=7)和室温(25℃)为最优条件.随着投菌量的增加,B.brevis对BaP的处理能力呈先增强后平缓的趋势;而BaP初始浓度的增加,则使降解量呈不断上升的趋势.水杨酸、琥珀酸和邻苯二甲酸的加入并不能明显提高B.brevis对BaP的降解效果.处理BaP后菌体表面褶皱增多,随着时间的延长,菌体出现表面凹陷、瘪塌变形的现象.
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| 关 键 词： | 苯并[a]芘 短短芽胞杆菌 筛选 生物降解 特性 |
| 收稿时间： | 2012/7/22 0:00:00 |
| 修稿时间： | 2012/10/25 0:00:00 |
Isolation of an Effective Benzo [a] pyrene Degrading Strain and Its Degradation Characteristics |
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| CAI Han,YIN Hu,YE Jin-shao,CHANG Jing-jing,PENG Hui,ZHANG Na and HE Bao-yan.Isolation of an Effective Benzo [a] pyrene Degrading Strain and Its Degradation Characteristics[J].Chinese Journal of Environmental Science,2013,34(5):1937-1944. |
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| Authors: | CAI Han YIN Hu YE Jin-shao CHANG Jing-jing PENG Hui ZHANG Na and HE Bao-yan |
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| Institution: | Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Department of Environmental Engineering, Jinan University, Guangzhou 510632, China;College of Environmental Science and Engineering, South China University of Technology, Guangzhou 510006, China;The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education,Guangzhou 510006, China;Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Department of Environmental Engineering, Jinan University, Guangzhou 510632, China;Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Department of Environmental Engineering, Jinan University, Guangzhou 510632, China;Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Department of Environmental Engineering, Jinan University, Guangzhou 510632, China;Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Department of Environmental Engineering, Jinan University, Guangzhou 510632, China;Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Department of Environmental Engineering, Jinan University, Guangzhou 510632, China |
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