| 二噁英类物质CALUX报告基因法在造纸行业的应用探究 |
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| 引用本文： | 陈燕,周志广,潘海婷,毕军平,秦迪岚,宋冰冰,谢沙,龙雯琪. 二噁英类物质CALUX报告基因法在造纸行业的应用探究[J]. 环境监测管理与技术, 2020, 32(2): 42-44. DOI: 10.3969/j.issn.1006-2009.2020.02.010 |
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| 作者姓名： | 陈燕 周志广 潘海婷 毕军平 秦迪岚 宋冰冰 谢沙 龙雯琪 |
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| 作者单位： | 湖南省生态环境监测中心,国家环境保护重金属污染监测重点实验室,湖南 长沙 410019,国家环境分析测试中心,北京 100029;国家环境保护二噁英污染控制重点实验室,北京 100029,湖南省生态环境监测中心,国家环境保护重金属污染监测重点实验室,湖南 长沙 410019,湖南省生态环境监测中心,国家环境保护重金属污染监测重点实验室,湖南 长沙 410019,湖南省生态环境监测中心,国家环境保护重金属污染监测重点实验室,湖南 长沙 410019,湖南省生态环境监测中心,国家环境保护重金属污染监测重点实验室,湖南 长沙 410019,湖南省生态环境监测中心,国家环境保护重金属污染监测重点实验室,湖南 长沙 410019,湖南省生态环境监测中心,国家环境保护重金属污染监测重点实验室,湖南 长沙 410019 |
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| 基金项目： | 全球环境基金资助项目(No. P125528) |
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| 摘 要： | 选择典型造纸厂,采用化学激活报告基因法(CALUX)测定造纸过程中废水和纸浆中的二噁英类物质,并将部分样品测定结果与高分辨气相色谱-高分辨质谱(HRGC-HRMS)法测定结果作线性回归,换算系数为0.21,表明CALUX可用于造纸行业废水中二噁英类物质的快速筛查。将CALUX用于测定造纸厂废水中二噁英类物质,结果表明用含元素氯的漂白工艺会产生大量二噁英类物质,其中漂白废水中的目标物毒性当量浓度最高,经处理后可达到国家排放标准;而用无元素氯的漂白工艺,二噁英类物质的产生量会大幅降低。
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| 关 键 词： | 二噁英 CALUX 高分辨气相色谱-高分辨质谱法 造纸行业 废水 |
Application of Chemical Activated Luciferase Gene Expression in Dioxins Detection in Papermaking Industry |
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| CHEN Yan,ZHOU Zhi-guang,PAN Hai-ting,BI Jun-ping,QIN Di-lan,SONG Bing-bing,XIE Sha,LONG Wen-qi. Application of Chemical Activated Luciferase Gene Expression in Dioxins Detection in Papermaking Industry[J]. The Administration and Technique of Environmental Monitoring, 2020, 32(2): 42-44. DOI: 10.3969/j.issn.1006-2009.2020.02.010 |
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| Authors: | CHEN Yan ZHOU Zhi-guang PAN Hai-ting BI Jun-ping QIN Di-lan SONG Bing-bing XIE Sha LONG Wen-qi |
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| Affiliation: | (Hunan Ecology and Environment Monitoring Center,State Environmental Protection Key Laboratory of Monitoring for Heavy Metal Pollutants,Changsha,Hunan 410019,China;National Research Center for Environmental Analysis and Measurement,Beijing 100029,China;State Environmental Protection Key Laboratory of Dioxin Pollution,Beijing 100029,China) |
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| Abstract: | Dioxins in pulping wastewater were detected by chemical activated luciferase gene expression(CALUX).Compared with high resolution gas chromatography-high resolution mass spectrometry(HRGC-HRMS),the results of some samples by the two methods were well correlated,the conversion coefficient was 0.21.The CALUX method could be applied in rapid screening for dioxins in pulping wastewater.In actual testing,large amount of dioxins were produced in the bleaching process with elemental chlorine,the toxic equivalent concentration of the target objects were the highest.After treatment,dioxins in the wastewater could meet the requirements of national discharge standard.The amount of dioxins would slash when using the bleaching process without elemental chlorine(ECF). |
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| Keywords: | Dioxins CALUX HRGC-HRMS Paper industry Waste water |
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