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餐厨降解菌的生物特性研究
引用本文:于鑫,邵晨,田兴军.餐厨降解菌的生物特性研究[J].环境科学学报,2014,34(7):1781-1787.
作者姓名:于鑫  邵晨  田兴军
作者单位:南京大学生命科学学院, 南京 210093;南京大学生命科学学院, 南京 210093;南京大学生命科学学院, 南京 210093
基金项目:国家水体污染控制与治理科技重大专项(No.2012ZX07204004-003);教育部博士点基金(No.20110091110018)
摘    要:为了研究餐厨降解菌的生物特性,选取耐盐性、基质迁移性及其在餐厨降解中的酶活特性展开研究,以此来揭示各菌种在降解过程中的优势特性及其各自发挥的作用.结果表明,混合菌产酶优于单菌种;菌株在2.5%NaCl浓度下可正常生长,耐盐性排序为:博德特氏菌(Bordetella petrii)汉氏硝化细菌(Nitrobacter hamburgensis)螺旋巴克斯霉(Backusella circina),迁移性排序为:博德特氏菌(Bordetella petrii)汉氏硝化细菌(Nitrobacter hamburgensis)螺旋巴克斯霉(Backusella circina).综合来看,博德特氏菌在降解前期作用明显,蔗糖酶活后期保持稳定表明堆肥矿质化趋于稳定,脱氢酶和多酚氧化酶活在后期逐步降低并趋于稳定表明堆肥腐熟性良好且稳定.

关 键 词:餐厨垃圾  微生物  耐盐性  迁移性  酶活
收稿时间:2013/10/15 0:00:00
修稿时间:2013/11/21 0:00:00

Biological characteristics of kitchen waste degrading bacteria
YU Xin,SHAO Chen and TIAN Xingjun.Biological characteristics of kitchen waste degrading bacteria[J].Acta Scientiae Circumstantiae,2014,34(7):1781-1787.
Authors:YU Xin  SHAO Chen and TIAN Xingjun
Institution:College of Life Science, Nanjing University, Nanjing 210093;College of Life Science, Nanjing University, Nanjing 210093;College of Life Science, Nanjing University, Nanjing 210093
Abstract:The present study was conducted to identify the dominant and effective utilization of kitchen garbage by different bacteria in compost. Salt tolerance, mobility and enzyme activity of bacteria were chosen as indicators of biological characteristics of kitchen garbage degradation. The results showed that enzyme production of mixed bacteria was better than single strain; bacteria tolerated 2.5% NaCl concentration in decreasing order of Bordetella petrii > Nitrobacter hamburgensis > Backusella circina while that of mobility decreased in order of Bordetella petrii > Nitrobacter hamburgensis > Backusella circina. In conclusion, Backusella circina played a vital role in the initial stage of degradation. The enzyme activity of invertase was stable in the late stage, suggesting that mineralization became stable during composting process. The enzyme activities of dehydrogenase and polyphenol oxidase decreased gradually and then stabilized in the late stage, indicating that maturity of composting was good and stable.
Keywords:kitchen garbage  microbe  salt tolerance  mobility  enzyme activity
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