CANNED工艺细菌种群结构变化的FISH研究

采用电镜观察和荧光原位杂交技术（FISH）,对亚硝化/电化学生物反硝化全自养脱氮（CANNED）工艺在不同游离氨（FA）质量浓度下反应器内细菌形态和种群结构进行了研究. 结果表明:在亚硝化段，ρ（FA）为0.5 mg/L时，氨氧化菌（AOB）和亚硝酸盐氧化菌（NOB）分别占总菌数的0.9%和2.8%；ρ（FA）为12.4～48.6 mg/L时，AOB和NOB分别占33.6%～47.4%和1.0%～2.9%，能够实现短程硝化. 在电化学生物反硝化段，当ρ（FA）为0.5～12.4 mg/L时，脱氮硫杆菌、厌氧氨氧化菌和真细菌分别占总菌数的7.2%～15.3%，7.2%～10.3%和9.1%～14.3%；当ρ（FA）增加到48.6 mg/L时，上述3种细菌所占比例分别增加到总菌数的34.5%，44.2%和60.8%，表明随着ρ（FA）的增加，提高ρ（FA）有利于上述3种菌的生长，与SEM结果一致. 

Study of Bacterial Community Changes in CANNED Process by FISH Method

Scanning electron microscope （SEM） and fluorescence in situ hybridization （FISH） were used to study the bacterial morphology and community of the complete autotrophic nitrite-nitrification and electrochemical bio-denitrification combined process （CANNED）, which was operated for ammonium wastewater treatment at different influent concentrations of free ammonia （FA）. When the mass concentration of FA was 0.5 mg/L in the nitrite-nitrification part, the AOB and NOB were 0.9% and 2.8% of the bacteria, respectively. It could achieve short-term nitrification when the mass concentration of FA was 12.4-48.6 mg/L. AOB and NOB were 33.6%-47.4% and 1.0%-2.9% of bacteria respectively under the above mentioned conditions. In the electrochemical bio-denitrification part, the thiobacillus denitrificans, anammox bacteria and eubacteria were 7.2%-15.3%, 7.2%-10.3% and 9.1%-14.3% of total bacteria respectively when the influent FA was 0.5-12.4 mg/L. When the mass concentration of FA increased to 48.6 mg/L, the above three kinds of bacteria were 34.5%, 44.2% and 60.8% of total bacteria respectively, which indicated that the growth of the bacteria was accelerated with the concentration of FA increasing. The above mentioned results were in accordance with the SEM results.