| 时间分辨荧光免疫一步法检测微囊藻毒素的研究 |
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| 引用本文： | 雷腊梅,韩博平,宋立荣.时间分辨荧光免疫一步法检测微囊藻毒素的研究[J].环境科学,2007,28(4):872-875. |
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| 作者姓名： | 雷腊梅 韩博平 宋立荣 |
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| 作者单位： | 1. 暨南大学水生生物研究中心,广州,510632
2. 中国科学院水生生物研究所,武汉,430072 |
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| 基金项目： | 广东省自然科学基金项目（5300418）;国家高技术研究发展计划（863）项目（2005AA6010100502）;暨南大学引进优秀人才科研启动基金项目 |
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| 摘 要： | 利用稀土元素Eu3+ 标记微囊藻毒素偶联物MCLR-BSA,在固相包被二抗的微孔板上建立了微囊藻毒素的直接竞争时间分辨荧光免疫法.条件优化后Eu3+ 标记物的稀释度为1∶50,微囊藻毒素单抗的合适浓度为100 ng/mL.该法对微囊藻毒素检测的灵敏度为0.02 ng/mL,试剂的测量范围是0.05～10 ng/mL,回收率达到94％以上.
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| 关 键 词： | 微囊藻毒素 时间分辨荧光免疫分析 检测 |
| 文章编号： | 0250-3301（2007）04-0872-04 |
| 收稿时间： | 2006/8/29 0:00:00 |
| 修稿时间： | 2006-08-292006-09-22 |
One Step Time-Resolved Fluorescence Immunoassay for Direct Estimation of Microcystins |
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| LEI La-mei,HAN Bo-ping and SONG Li-rong.One Step Time-Resolved Fluorescence Immunoassay for Direct Estimation of Microcystins[J].Chinese Journal of Environmental Science,2007,28(4):872-875. |
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| Authors: | LEI La-mei HAN Bo-ping and SONG Li-rong |
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| Institution: | 1.Research Center of Hydrobiology, Jinan University, Guangzhou 510632, China; 2.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China |
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| Abstract: | A direct-competitive time-resolved fluorescence immunoassay(TRFIA) for microcystin detection was established,which was based on europium labeled MCLR-BSA conjugant and microtiter plates coated with anti-mouse IgG.The optimal dilution of europium labeled MCLR-BSA conjugant is(1/50) and most appropriate titration of anti-microcystin-LR(MCLR) monoclonal antibodies is 100 ng/mL.The standard curve under the optimal conditions shows that the quantitative range is from approximately 0.05(ng/mL) to 10(ng/mL) The detection limit of the method is 0.02(ng/mL) for MCLR,recoveries for microcystin(>94%) in quantitative range is satisfactory. |
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| Keywords: | microcystin time-resolved fluorescence immunoassay detection |
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