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邻苯二甲酸二丁酯对拟南芥幼苗的氧化损伤(Oxidative Damage Induced by Dibutyl Phthalate to Stem and Leaves of Arabidopsis Seedlings)
引用本文:陈晓晓,张雯,武阳,杨旭.邻苯二甲酸二丁酯对拟南芥幼苗的氧化损伤(Oxidative Damage Induced by Dibutyl Phthalate to Stem and Leaves of Arabidopsis Seedlings)[J].生态毒理学报,2010,5(3):402-406.
作者姓名:陈晓晓  张雯  武阳  杨旭
作者单位:华中师范大学生命科学学院,环境科学实验室和湖北省遗传调控与整合生物学重点实验室,武汉,430079
基金项目:国家自然科学基金面上项目(No. 51076079)
摘    要:为了探讨邻苯二甲酸二丁酯(DBP)对植物的氧化损伤作用,采用不同浓度的DBP溶液对拟南芥(Arabidopsis thaliana)进行染毒(终浓度为0、0.5、2.5、5、10mg·L-1),检测DBP对幼苗茎、叶超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的影响.结果表明:染毒1周后,随DBP浓度的升高,拟南芥幼苗茎叶MDA含量呈显著上升趋势,高浓度组(5、10mg·L-1)与对照组差异显著(p<0.01);SOD活性随DBP浓度的升高呈先升高后降低趋势,各染毒组与对照组均差异显著(p<0.01).以上结果表明DBP可对拟南芥幼苗产生明显的氧化损伤.

关 键 词:邻苯二甲酸二丁酯  拟南芥  生态毒性  丙二醛  超氧化物歧化酶
收稿时间:2009/12/8 0:00:00
修稿时间:2010/1/15 0:00:00

Oxidative Damage Induced by Dibutyl Phthalate to Stem and Leaves of Arabidopsis Seedlings
CHEN Xiao-xiao,ZHANG Wen,WU Yang and YANG Xu.Oxidative Damage Induced by Dibutyl Phthalate to Stem and Leaves of Arabidopsis Seedlings[J].Asian Journal of Ecotoxicology,2010,5(3):402-406.
Authors:CHEN Xiao-xiao  ZHANG Wen  WU Yang and YANG Xu
Abstract:In order to explore the oxidative damage induced by dibutyl phthalate(DBP)to plants,the Arabidopsis thaliana was exposed to different concentrations of DBP(the terminal concentrations were 0,0.5,2.5,5 and 10mg·L-1),and the malondialdehyde(MDA) content and superoxide dismutase(SOD) activity in stem and leaves were determined.Results showed that the MDA content in stem and leaves increased gradually with the increase of DBP concentrations after exposed to DBP for a week,and the MDA content in high concentration groups(5,10mg·L-1) was significantly higher than that in the control(p<0.01).The SOD activity in stem and leaves increased first and then decreased with the increase of DBP concentrations,and the SOD activity in all exposure groups was significantly higher than that in the control(p<0.01).Above results suggested that DBP could induce oxidative damage to Arabidopsis seedlings.
Keywords:dibutyl phthalate(DBP)  Arabidopsis  ecological toxicity  malondialdehyde(MDA)  superoxide dismutase(SOD)
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