子牙河水系水和沉积物好氧氨氧化微生物分布特征

采集海河流域子牙河水系河流沉积物和河流水样,分析了其好氧氨氧化微生物的分布特征,并探讨了氨氮、溶解氧和pH对其分布的影响.结果表明,沉积物中氨氧化古菌（AOA）和氨氧化细菌（AOB）丰度范围分别为1.05×105～1.18×109genecopies·g-1和1.04×105～2.46×109genecopies·g-1,水体AOA和AOB丰度范围分别为5.21×102～2.44×109genecopies·mL-1和1.75×102～1.56×1010genecopies·mL-1;沉积物中AOB占优势,平均丰度为AOA的8.51倍,水中AOA占优势,平均丰度为AOB的18.99倍.偏相关分析表明,氨氮浓度同水中AOB与AOA丰度比值显著正相关（r=0.477,p〈0.05）,pH同水体AOB丰度显著正相关（r=0.466,p〈0.05）,而溶解氧同水体AOB丰度及AOB、AOA丰度比值都显著正相关（r分别为0.722和0.745,p〈0.01）.

The distribution of aerobic ammonia oxidizing microorganisms in Ziya River, Haihe Basin

Surface sediment and water column samples of Ziya River in Haihe Basin was collected to analyze the effect of environmental parameters such as ammonium, dissolved oxygen, and pH to the distribution of aerobic ammonia oxidizing microorganisms. The results showed that the average abundance of ammonia oxidizing bacteria (AOB) (ranging from 1.04×10⁵ to 2.46×10⁹ gene copies·g^-1) in sediment was 8.51 times that of ammonia oxidizing archaea (AOA) (ranging from 1.05×10⁵ to 1.18×10⁹ gene copies·g^-1). While in river water column, the average abundance of AOB (ranging from 1.75×10² to 1.56×10¹⁰ gene copies·mL^-1) was nearly a twentieth part of AOA (ranging from 5.21×10² to 2.44×10⁹ gene copies·mL^-1). Partial correlation analysis indicated that ammonia concentration was positively correlated with the AOB-to-AOA ratio (r=0.477, p<0.05) and pH was positively correlated with AOB abundance (r=0.466, p<0.05) in river water column. Moreover, dissolved oxygen showed positive relationship with both the AOB-to-AOA ratio and AOB abundance, with the correlation coefficients of 0.722 and 0.745, respectively (p<0.01).