菌株HP3对溴胺酸的降解机理

通过正交试验,得到菌株HP3降解溴胺酸最佳条件为温度30℃,pH值7.0,摇床转速160r/min,培养基中不含NaCl有利于溴胺酸的降解.在此条件下,溴胺酸的降解遵从负指数模式.葡萄糖和硫酸铵的加入显著地提高菌体降解溴胺酸的速度.不同金属化合物对菌株HP3降解溴胺酸有不同程度的抑制作用,其中以HgCl₂和AgNO₃最显著.菌株HP3能降解化合物蒽醌、1,4,5,8-四羟基蒽醌和1-氨基蒽醌-2-磺酸钠,表明该菌株对底物的降解没有严格的专一性.溴胺酸经菌株HP3作用后吸收光谱发生明显变化.TOC分析表明,菌株HP3将溴胺酸的蒽醌环破坏后利用其中的一部分有机碳.液相色谱检测有中间产物邻苯二甲酸生成.溴胺酸降解终产物分子量为289和290.

Degradation and its mechanism of 1-amino-4-bromoanthraquinone-2-sulfonic sodium by strain HP3.

The optimal degradation conditions of 1-amino-4-bromoanthraquinone-2-sulfonic sodium(ABAS) by strain HP3 temperature=30℃, pH=7.0, speed of shake-bed=160r/min and NaCl=0 in the culture basis were obtained by orthogonal test. Under these conditions, the ABAS degradation followed negative exponential model. The addition of glucose and (NH4)2SO4 could greatly enhance the degradation rate of ABAS by the strain. All experimental metal compounds restrained the degradation of ABAS in different degrees, in which HgCl2 and AgNO3 could control the degradation most obviously. Strain HP3 also degraded anthraquinone, 1,4,5,8-tetrahydroanthraquinone and 1-animoanthraquinone 2-sulfonic sodium. Degraded by HP3, the absorption spectrum of ABAS changed greatly showing that the degradation was not concentrated on single basis. TOC analysis showed a part of organic carbon from destroyed anthraqui none of ABAS was utilized; and the medial prduct o-phthalic acid formed as determined in liquid chromospreetrum. The molecule weights of the final products from the ABAS degradation were 289 and 290.