复合微生物絮凝剂产生菌XL1的优化条件

从活性污泥中筛选出4株产微生物絮凝剂的菌株，将4株菌进行相互复合培养后，发现1号和2号菌株构建的复合菌群（XL1）所产MBF的絮凝率达到65．5％。优化XLI的培养条件及其所产MBF的絮凝条件后，发现XL1在培养温度为35℃、摇床转速为160rpm、培养基初始pH为8．0、菌液用量为15ml／L、种子液接种量为10ml／L、1％CaCl，溶液用量为40ml／L、高岭土悬浮液pH为4．5、静沉时间为12min等的条件下絮凝率可达到92．5％。16S rDNA测序鉴定1号菌属于沙雷菌属（Serratia），2号菌属于芽孢杆菌菌属（Bacillus）。

Optimum Conditions of Compound Bioflocculation Producing Bacterium XL1

Four bacterial stmins were screened from active sludge, after four stmins of bacteria were combined with each other, it was found that flocculating efficiency of multiple microorganisms with No. 1 and No. 2 was up to 65. 5%. After optimizing culture, it indicated that the optimum culture conditions were as follows： the temperature was 35℃, the rotation rate was 160r/min, the initial pH of medium was 8. 0, the amount of bacteria was 15ml/L, the amount of seed solution was 10ml/L, the amount of CaC12 was 40ml/L, the initial pH of Kaolin suspension was 4. 5, the time of standing was 12min. Under these optimum culture conditions, the flocculation rate was up to 92. 5%. It illustrated that the bacteria No. 1 was identified was Serratia and the bacteria No. 2 was identified as Bacillus by sequencing of 16SrDNA.