| 高脂肪样品中PCBs和OCPs分离方法研究 |
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| 引用本文： | 宫福强,;包姣灵,;王迎春,;吕晓宁,;孙梦雪,;李清波.高脂肪样品中PCBs和OCPs分离方法研究[J].国外农业环境保护,2014(5):461-469. |
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| 作者姓名： | 宫福强 ;包姣灵 ;王迎春 ;吕晓宁 ;孙梦雪 ;李清波 |
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| 作者单位： | [1]大连市环境监测中心,辽宁大连116026; [2]大连海事大学环境科学与工程学院,辽宁大连116026 |
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| 基金项目： | 国家自然科学基金资助项目（40671166,41371448） |
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| 摘 要： | 研究开发了用于分离高脂肪样品中21种多氯联苯（PCBs）和22种有机氯农药（OCPs）的分离柱填料,以及利用该填料分离含脂肪的动物组织样品中PCBs或OCPs的方法。该填料按质量百分比由硅胶-弗罗里硅土混合物30%-35%、酸性改性硅胶50%-60%和无水硫酸钠10%-15%组成,并使用自主研发的四通道色谱分离仪对样品进行分离和净化。利用本方法的填料分离PCBs或OCPs,分离方法高效、快速、工艺简单,可在成本较低的条件下达到显著的分离效果。PCBs化合物柱回收率可达96.4%-119%,对鱼脂肪组织加标回收率可达74.4%-100%;OCPs化合物柱回收率可达78.4%-103%,对鱼脂肪组织加标回收率可达78.3%-102%。结果表明该填料可用于分离PCBs或OCPs,色谱分离效果良好,可以满足高脂肪样品中OCPs和PCBs的监测分离需要。
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| 关 键 词： | 填料 多氯联苯 有机氯农药 分离方法 |
A Method for Separating PCBs and OCPs in Biota Samples |
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| Institution: | GONG Fu-qiang, BAO Jiao-ling, WANG Ying-chun, LU Xiao-ning, SUN Meng-xue, LI Qing-bo (1.Dalian Environmental Monitoring Center, Dalian 116026, China; 2.Department of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China) |
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| Abstract: | Chromatographic fraction and cleanup method was developed for PCBs and OCPs in biota samples, using a self-developing chromatographic fraction instrument and solid phase mixture. The solid phase was composed of florisil（30%-35%）, acid-treated silica gel（50%-60%）and anhydrous sodium sulphate（10%-15%）. The recoveries of spiked PCBs and OCPs in column ranged from 96.4% to 119% and from78.4% to 103% respectively, while in fish fat tissue ranged from 74.4% to 100% and from 78.3% to 102% respectively. This approach was proved to be an efficient, fast, simple and cost-effective method for fraction and cleanup of PCBs and OCPs in biota samples. |
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| Keywords: | solid phase polychlorinated biphenyls organochlorine pesticides fraction |
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