亚硝态氮和氨态氮急性胁迫下曼氏无针乌贼幼体血液的生化指标

将曼氏无针乌贼幼体置于24 h半致死剂量的亚硝态氮和氨态氮溶液中(10 mg·L-1 NaNO2和300 mg·L-1 NH4Cl),检测6、12和24 h各点及对照组曼氏无针乌贼幼体血液生化指标的变化.结果表明,随着处理时间的延长,NaNO2处理组血细胞密度(THC)(p＜0.05)、甘油三酯(TG)(p＜0.05)...

Biochemical Parameters in Juvenile Sepiella maindroni Induced by Acute Exposure to Nitrite-N or Ammonia-N

To better understand the effects of nitrite-N and ammonia-N on blood of the juvenile Sepiella maindroni, Juvenile Sepiella maindroni were exposed to 10 mg·L^-1 sodium nitrite or 300 mg·L^-1 ammonium chloride for 0, 6, 12 and 24 hours respectively. The blood immune parameters-total haemocyte count (THC), glucose (GLU), cholesterol (CH), triglyceride (TG), total protein (T-MP) concentrations, glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT) activities, GOT/GPT ratios were investigated in this study. The results show that THC (p<0.05), TG concentration (p<0.05) and GPT activity (p<0.05) first fell then rose, running counter to that of GOT activity (p<0.05) and GOT/GPT ratio (p<0.05); GLU concentration (p>0.05) first rose then fell and rose again at 24 h; CH concentration (p>0.05) decreased significantly, running counter to that of T-MP (p<0.05) with the elapse of nitrite-N exposure time. With the elapse of ammonia-N exposure time, THC (p<0.05), GPT (p<0.05) and GOT activities (p<0.05) decreased significantly, running counter to that of GOT/GPT ratio (p<0.05); GLU concentration (p<0.05) first fell then rose and fell again at 24 h, running counter to that of CH (p<0.05), TG (p<0.05) and T-MP (p<0.05). The results suggest that the differences of blood biochemical parameters between nitrite-N and ammonia-N result from their characteristics. Liver was more seriously damaged caused by nitrite-N than ammonia-N, however, that was not the main reason for death. Protein and GLU were complementary in eliminating ammonia-N, and which can be used as biomarkers of exposure to ammonia-N with CH and TG.