沿海三市饮用水源水内分泌干扰毒性研究

近十多年来,江苏沿海化工产业发展迅速,化工废水的长期排放对水生生态系统及人群健康构成潜在威胁.采用非洲猴肾细胞(CV-1)核受体介导的体外转录激活试验方法,对中国东部沿海A、B、C三市的6个水源地进行了拟雌激素活性调查研究.结果表明:C市2处水源水的有机提取物在枯水期、平水期和丰水期均无拟雌激素活性检出,水质较好；A市...

Study on Endocrine-Disrupting Toxicity in Drinking Water Sources of Three Coastal Cities

Chemical industries have developed rapidly. Over the last decade, along the sea in Jiangsu Province, chemical wastewater discharged in long-term made potential threats to the ecosystem and human health. In this study, ER-mediated reporter gene assay was applied in evaluating the endocrine-disrupting activity of surface water from 6 water sources in 3 coastal cities of Jiangsu Province. The results indicated that source water in city C was the cleanest. Water extracts from city A in dry season could induce significant estrogenic activity. Extracts from water sources in city B could induce significant estrogenic activity in all three sampling seasons. Estrogenic activity equivalent at location B-3 in the dry season was higher than the indicated toxicity limit, which may pose a threat to the aquatic organism. More attentions should be paid to this part. This study also showed that reporter gene assay depending on CV-1 cell line was an efficient and rapid method for screening and analyzing of environmental samples, which worth further promotion and application.