营养源对活性艳红脱色降解体系的影响研究

选择活性艳红X-3B为脱色和降解对象，将白腐真菌固定在生化填料上构建白腐真菌生物膜反应器，并以接种活性污泥的生物膜反应器为对照，考察了营养源对活性艳红脱色降解的影响。实验表明，对白腐真菌Shu-13采用查氏培养基预培养和在反应器中投加适量蔗糖及酱油废水，均能明显提高活性艳红X-3B的脱色率。城市污水中的营养源，不足以满足白腐真菌对活性艳红X-3B的脱色降解需要，白腐真菌Shu-13对富氮条件存在依赖性。在富营养条件下，白腐真菌Shu-13生物膜反应器对活性艳红X-3B的脱色效果明显优于活性污泥。营养源及其浓度、菌种是影响pH变化的重要因素，活性污泥和酱油曲霉生物膜反应器的出水pH变化规律，与Shu-13存在明显不同，Shu-13对活性艳红X-3B的脱色过程中发生了明显的降解作用，而这种作用对生物膜恢复其污染物净化功能是至关重要。另外，酱油废水中存在的酱油曲霉属于半知菌纲的白腐真菌，其对活性艳红X-3B也有较显著的脱色降解效果。

Study on Effects of Nutrients on Decolorization and Degradation of Reactive Brilliant Red X-3B by Using White Rot Fungal Technology

Selecting reactive brilliant red X-3B(RBRX-3B)as the object of decolorization and degradation, and con-structing white rot fungal biofilm reactor by using white rot fungus immobilized on biochemical filler, and taking activated sludge biofilm reactor as control, to observe the effect of nutrients on decolorization and degradation of RBRX-3B.The experiment showed that nutritional enrichment could greatly enhance RBRX-3B decolorization by white rot fungus Shu-13.The nutrients in municipal sewage is not enough to meet the necessity of white rot fungus for decolorizing and degrading RBRX-3B,for white rot fungus Shu-13 is dependent on the presence of rich nutrition. Under rich nutrient condition, the decolorization efficiency of white rot fungus Shu-13 biofilm to RBRX-3B was obviously better than activated sludge. Nutrient and its concentration, microbial species were the important factors to affect change of pH. The law of pH change of flow water from activated sludge and A. sojae biofilm reactor differed obviously from Shu-13. Dur-ing decolorization of RBRX-3B, Shu-13 played a remarkably role on degradation, while biofilm recovered its pollutant purification capatility was most important.