| 二苯并噻吩降解菌筛选及16S rDNA序列分析 |
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| 引用本文： | 金义鑫,杨艳燕,袁琳,刘红海,沈轶,李亚东.二苯并噻吩降解菌筛选及16S rDNA序列分析[J].环境科学与技术,2006,29(3):19-20. |
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| 作者姓名： | 金义鑫 杨艳燕 袁琳 刘红海 沈轶 李亚东 |
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| 作者单位： | 湖北大学生命科学学院,武汉,430062 |
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| 基金项目： | 湖北省自然科学基金
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湖北省重点实验室基金 |
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| 摘 要： | 以DBT(dibenzothiophene,二苯并噻吩)为模型化合物,分离得到了一株能降解DBT的微生物HB2,应用HPLC对其脱硫特性进行了检测。应用PCR技术克隆到16SrDNA片段,核苷酸序列分析结果表明,该菌的16SrDNA全序列与假单胞菌存在98%的同源性,初步确定该菌在微生物系统发育学上的地位。
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| 关 键 词： | 二苯并噻吩 微生物脱硫 16SrDNA |
| 文章编号： | 1003-6504(2006)03-0019-02 |
| 收稿时间： | 2005-02-15 |
| 修稿时间： | 2005-04-17 |
Isolation of Dibenzothiophene-degrating Strain and Its 16S rDNA Sequence Analysis |
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| JIN Yi-xin,YANG Yan-yan,YUAN Lin,LIU Hong-hai,SHEN Yi,LI Ya-dong.Isolation of Dibenzothiophene-degrating Strain and Its 16S rDNA Sequence Analysis[J].Environmental Science and Technology,2006,29(3):19-20. |
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| Authors: | JIN Yi-xin YANG Yan-yan YUAN Lin LIU Hong-hai SHEN Yi LI Ya-dong |
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| Abstract: | Dibenzothiophene(DBT),the typical compound of sulfur-containing organic molecules found in fossil fuels,can be utilized by a strain called HB2,which was isolated in the lab.PCR technology was then used to amplify its 16S ribosomal DNA,i.e.,rDNA,which was found to be homogeneous as much as 98% to Pseudomonas;therefore,basically the position of the strain in microbiological phylogeny was set up. |
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| Keywords: | dibenzothiophene desulfurization by microbe 16S rDNA |
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