In vitro response of human ovarian cancer cells to dietary bioflavonoid isoquercitrin |
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| Authors: | Katarina Michalcova Shubhadeep Roychoudhury Marek Halenar Eva Tvrda Eva Kovacikova Jaromir Vasicek |
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| Institution: | 1. Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic;2. Department of Life Science and Bioinformatics, Assam University, Silchar, India;3. Research Centre AgroBioTech, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic;4. Research Institute for Animal Production Nitra, National Agricultural and Food Centre, Lu?ianky, Slovak Republic |
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| Abstract: | AbstractIsoquercitrin is a dietary bioflavonoid used as a food supplement. We studied the mechanism underlying its effect in human ovarian cancer cells using OVCAR-3 cell line. Viability, survival, apoptosis, release of human transforming growth factor-β1 (TGF-β1) and TGF-β1 receptor, and intracellular reactive oxygen species (ROS) generation by OVCAR-3 cells were examined after isoquercitrin treatment at concentrations 5, 10, 25, 50, and 100?μg mL?1. AlamarBlue assay revealed that isoquercitrin did not cause any significant change (P?>?0.05) in cell viability as compared to control. Apoptotic assay using flow cytometry did not find any significant change (P?>?0.05) in the proportion of live, dead and apoptotic cells as compared to control. ELISA also showed that the release of human TGF-β1 and TGF-β1 receptor were not significantly (P?>?0.05) affected by isoquercitrin as compared to control. Chemiluminescence assay demonstrated that lower concentrations (5, 10, and 25?μg mL?1) were able to exhibit beneficial effects by inhibiting the generation of intracellular ROS. In contrast, elevated concentrations of 50 and 100?μg mL?1 led to oxidative stress (P?
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| Keywords: | Isoquercitrin human ovarian cancer cell viability apoptosis TGF-β1 ROS |
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