镉铜污染尾矿土中添加耐镉铜菌剂J5后微生物区系多样性的变化

采用酶解法和化学裂解法相结合的方法从安徽铜陵镉铜污染尾矿土中提取出土壤微生物基因组总DNA,并应用PCR-DGGE技术对比研究尾矿土中添加外源硫酸镉、硫酸铜和耐镉铜菌剂J5(Pseudomonassp.)后微生物区系多样性的变化.结果表明,尾矿土中微生物基因组总DNA分子量大小为21kb左右,未复垦的尾矿砂中微生物总DNA量较少,CdSO4处理后的尾矿土中微生物基因组总DNA量明显下降,而添加菌剂J5的尾矿土中微生物基因组总DNA量上升.DGGE电泳图谱进一步显示,尾矿土样中添加镉离子使图谱条带减少,分析表明尾矿土中的细菌种类数减少;而添加菌剂J5使图谱中条带明显增加,表明菌剂J5可提高尾矿土中土著微生物的活性和群落丰富度,可用于复合重金属污染尾矿区的生态恢复.

Study on the Change of Microbial Community Diversity in Contaminated Soils of Cadmium-Copper Mine Tailings after the Tolerant-Cadmium and Tolerant-Copper Strain J5 Added

This paper was on the change of microbial community diversity after CdSO4 and CuSO4 and the tolerant-cadmium and tolerant-copper strain J5(Pseudomonas sp.)added in cadmium-copper contaminated mine tailings in Tongling in Anhui province by PCR-DGGE. General microorganism DNA could be extracted directly from the tailing soil samples by the enzyme-chemistry method. The results showed that the size of DNA was about 21kb. The DNA content was little in mine soil without any plant covered and the content of general DNA would decline in the tailing soil treated by CdSO4. While the content of general DNA would rise by the tolerant-cadmium and tolerant-copper strain J5 ferment liquid added in the tailing soils. DGGE electrophoresis figure showed that the strips reduced treated by CdSO4. It was implied the number of bacteria reduced. While strain J5 was added in the tailing soils, it could improve native microbial activity and community richness. The decline of soil microbial community functional diversities would be prevented in polluted soils. Our study provides an available approach to quickly resume mine-tailings areas contaminated by multi-heavy metal with strain J5 added.