| 联苯胺对鲫鱼肝线粒体DNA(mtDNA)作用的研究 |
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| 引用本文： | 刘辉,刘伟民,周培疆.联苯胺对鲫鱼肝线粒体DNA(mtDNA)作用的研究[J].环境科学与技术,2011,34(8):18-21. |
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| 作者姓名： | 刘辉 刘伟民 周培疆 |
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| 作者单位： | 1. 环境保护部华南环境科学研究所,广东广州,510655
2. 武汉大学,湖北武汉,430000 |
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| 基金项目： | 国家自然科学基金项目(40801086) |
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| 摘 要： | 采用平板电泳的方法,研究等浓度连续喂养的鲫鱼肝线粒体DNA的表达条带差异特征.结果表明:该实验中的鲫鱼mtDNA拥有两个EcoR Ⅰ的酶切位点;随着联苯胺作用时间的延长,mtDNA双链断裂呈三个依次递进的状态;线粒体DNA的断裂总是从低分子量链向高分子量链的断裂转移.
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| 关 键 词： | 联苯胺 鲫鱼肝mtDNA 体外染毒 电泳 |
MtDNA of Crucian's Liver by Benzidine |
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| LIU Hui,LIU Wei-min,ZHOU Pei-jiang.MtDNA of Crucian's Liver by Benzidine[J].Environmental Science and Technology,2011,34(8):18-21. |
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| Authors: | LIU Hui LIU Wei-min ZHOU Pei-jiang |
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| Institution: | LIU Hui1,LIU Wei-min1,ZHOU Pei-jiang2(1.South China Institute of Environmental Science,Ministry of Environment Protection,Guangzhou 510655,China,2.Wuhan University,Wuhan 430000,China) |
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| Abstract: | Technique of flat electrophoresis was used to study the difference characteristics of mtDNA of crucian's liver that continuously feed with the same concentration of benzidine.Results showed that mtDNA of crucian had two endonuclease sites of EcoR I,and the damage of benzidine to mtDNA began from lower molecular weight.With the time prolonged,the snippets of mtDNA were increased at lower molecular weight,and the molecular weight of snippets became lower. |
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| Keywords: | benzidine mtDNA of crucian's liver toxicity out vitro flat electrophoresis |
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