耐铅、铜微生物的筛选及吸附性能研究

采用浓度梯度筛选的方法，从重金属污染土壤中分离筛选到两株耐受和吸附铅、铜的细菌G、Y。用单因素的方法得到G最适的培养条件为．T=30℃；pH=6．O；NaCl浓度为0．5％；Y的最适培养条件分别为：T=37℃；pH=8．5；NaCl浓度为0．5％。用原子吸收分光光度法测得，G、Y的冻干菌体对Pb2＋、Cu2＋的吸附量分别为：G铅 126．42mg／g，G铜75mg／g，Y钢61．08mg／g，Y钢45．86mg／g。G湿菌体对铅、铜的吸附率分别为：G铅94．66％，G铜92．84％；Y湿菌体对铅、铜的吸附率分别为：Y铅99．74％，Y铜97．96％。经检测，其遗传性状稳定。可进一步开发为重金属吸附剂。

Study on Screening of Tolerant-Lead & Copper Strains and Its Adsorption Capability

Two strains capable of tolerating and adsorbing Lead and Copper,was isolated from heavy metals-contaminated soils through concentration gradient screening technique,named G & Y.By using the single factor method,the optimum growth conditions were determined,they were:G∶T30℃,pH6.0,NaCl concentration 0.5%,Y∶T37℃,pH8.5,NaCl concentration 0.5%.Measured and analyzed by Determination Method-Atomic Absorption Spectrophotometry,Pb2+、 Cu2+ could be adsorbed by freeze dried strains,the adsorption capacity of them are GPb 126.42mg/g,GCu 75 mg/g,YPb 61.08 mg/g,YCu 45.86 mg/g.Pb2+&Cu2+ could also be adsorbed by waterish strains,the adsorption rate of G were,GCu92.84%,GPb94.66%,the adsorption rate of Y were∶YCu97.96%,YPb99.74%.They were measured stable.And can be developed as sorbents for bioremediation of heavy metals.