十六烷基三甲基氯化铵抑制小球藻生长的效应及作用机制

以普通小球藻为受试生物, 采用批量培养方法研究了阳离子表面活性剂十六烷基三甲基氯化铵(CTAC)的抑藻效应,通过营养元素吸收、Zeta电位、酸性磷酸酶和亚显微结构的测定探讨了其作用机制. 结果表明, CTAC浓度在0.1～1 mg/L范围内随浓度升高对小球藻的抑制作用增强, 96 h抑制小球藻生长的半效应浓度(96 h-EC₅₀)为0.18 mg/L. 0.3 mg/L CTAC作用8 d后, 对小球藻生物量的抑制率为70.7%, 对氮和铁单位吸收量的抑制率分别为83.9%和86.2%, 藻细胞Zeta电位从-12.5 mV提高至-6.7 mV, 酸性磷酸酶相对活力降至23.1%, 藻细胞出现明显的质壁分离、蛋白核扭曲和溶酶体膨胀等现象. 根据实验结果分析, CTAC提高了藻细胞Zeta电位，抑制了小球藻对氮和铁的吸收, 影响磷的代谢并引起亚显微结构的改变.

Growth Inhibition and Mechanism of Cetyltrimethyl Ammonium Chloride on Chlorella vulgaris

Growth inhibition of cetyltrimethyl ammonium chloride(CTAC),a cationic surfactants,on Chlorella vulgaris was investigated at batch culture in laboratory.Furthermore,the corresponding mechanisms were studied by the determination of absorption capacity,Zeta potential,activity of acid phosphatase and ultrastructure of algae.Results show that the growth inhibition by CATC is enhanced with its concentration increasing from 0.1(mg/L) to 1(mg/L),and 96 h-EC_(50) of CTAC is 0.18(mg/L).In the presence of 0.3(mg/L) C...