Noninvasive molecular genetic analysis in studying the ecology of the snow leopard: Problems and prospects |
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Authors: | V. V. Rozhnov E. Yu. Zvychainaya A. N. Kuksin A. D. Poyarkov |
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Affiliation: | 1.Severtsov Institute of Ecology and Evolution,Russian Academy of Sciences,Moscow,Russia;2.Ubsunurskaya Kotlovina State Nature Biosphere Reserve,Kyzyl,Republic of Tyva, Russia |
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Abstract: | Molecular genetic methods were used to perform the species and individual identification of 117 samples of hair and feces of carnivores collected in natural habitats. The species were identified for 85.5% of the samples. The analysis of the nucleotide sequences of the cytochrome b mitochondrial gene was used to identify the samples of hair and feces of snow leopards (Uncia uncia, 40%), red foxes (Vulpes vulpes, 48%), wolves (Canis lupus, 6%), and lynx (Lynx lynx, 5%). The analysis of eight microsatellite loci allowed the genotypes of 13 snow leopards to be described. The sex of 11 animals was identified. |
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