| 隐孢子虫3种检测方法比较 |
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| 引用本文： | 李绍峰,冉治霖,高云霞,黄君礼,崔崇威. 隐孢子虫3种检测方法比较[J]. 环境工程学报, 2009, 3(1): 47-51 |
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| 作者姓名： | 李绍峰 冉治霖 高云霞 黄君礼 崔崇威 |
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| 作者单位： | 深圳职业技术学院建筑与环境工程学院,深圳,518055;哈尔滨工业大学环保科技有限公司博士后工作站,哈尔滨,150001;哈尔滨工业大学市政环境工程学院,哈尔滨,150090 |
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| 基金项目： | 国家“863”高技术研究发展计划项目（2006AAZ309） |
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| 摘 要： | 为建立一套行之有效的隐孢子虫灭活效果评价方法,在构建鸡源隐孢子虫C. baileyi动物模型的基础上,选用3种检测方法（抗酸染色、饱和蔗糖悬浮镜检和荧光检测）,对鸡源隐孢子虫进行镜检、数量统计,并比较3种方法的优劣及适用范围。结果显示：荧光染色法具有最佳的检测效率（检出数量为改良抗酸染色法,饱和蔗糖悬浮镜检法的2.1和2.6倍;检测时间为120 min）,并能够进行隐孢子虫的活性鉴定,可以作为饮用水消毒剂灭活隐孢子虫的评定方法,是一种高效、简便、较为廉价,并具有较大发展前景的检测方法。
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| 关 键 词： | 隐孢子虫 荧光染色 |
Comparison of three methods for detection of Cryptosporidium |
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| Li Shaofeng,Ran Zhilin,Gao Yunxi,Huang Junli and Cui Chongwei. Comparison of three methods for detection of Cryptosporidium[J]. Techniques and Equipment for Environmental Pollution Control, 2009, 3(1): 47-51 |
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| Authors: | Li Shaofeng Ran Zhilin Gao Yunxi Huang Junli Cui Chongwei |
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| Affiliation: | 1.Department of Architecture Engineering, Shenzhen Polytechnic Institute, Shenzhen 518055;2. Research Station for Postdoctoral Fellows, Environmental Technology Co. Ltd., Harbin Institute of Technology, Harbin 150001,3.School of Municipal and Environmental Engineering,Harbin Institute of Technology, Harbin 150090,3.School of Municipal and Environmental Engineering,Harbin Institute of Technology, Harbin 150090,3.School of Municipal and Environmental Engineering,Harbin Institute of Technology, Harbin 150090 and 3.School of Municipal and Environmental Engineering,Harbin Institute of Technology, Harbin 150090 |
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| Abstract: | In order to construct an efficient method for evaluating disinfection effect of Cryptosporidium,based on establishing a Cryptosporidium bailey infective model in chick,three techniques were used for examining the quantity of C.bailey oocyst: acid-fast staining,saturated sucrose flotation inspection,fluorescence staining detection.The results show that fluorescence staining detection has the best measurement efficiency,the number of measured oocyst is 2.1 and 2.6 times of acid-fast staining and saturated-suc... |
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| Keywords: | Cryptosporidium fluorescence staining detection |
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