Supramolecular complex formation,a possible antigenotoxic mechanism of glucomannan against aflatoxin B1 |
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Authors: | Eduardo Madrigal-Santillán Eduardo Madrigal-Bujaidar Alicia Reyes-Arellano José Antonio Morales-González Isela Álvarez-González Manuel Sánchez-Gutiérrez |
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Institution: | 1. Escuela Superior de Medicina, Instituto Politécnico Nacional, Unidad Casco de Santo Tomas, Ciudad de México, México;2. eomsmx@yahoo.com.mx;4. Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional A. López Mateos, Ciudad de México, México;5. Departamento de Química Orgánica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Casco de Santo Tomas, Ciudad de México, México;6. Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Hidalgo, México |
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Abstract: | AbstractGlucomannan is a highly branched polysaccharide with glycosidic linkages, constituted of mannoses and glucoses. In recent years, its usefulness due to its immunological, antioxidant and antimutagenic activity has been recognized. The aim of the study was to determine the antigenotoxic ability of glucomannan extracted from Candida utilis orally administered (100–700?mg/kg) to mice, which subsequently received 1?mg/kg aflatoxin B1. Hepatocytes obtained from these animals 4–16?h post administration were examined by means of the comet assay. The antigenotoxic effect was found to be higher than that observed in previous studies with α-mannan and β-D-glucan isolated from Saccharomyces cerevisiae., In order to explore the possibility of formation of a supramolecular complex between glucomannan and aflatoxin B1, both compounds were co-crystallized, their melting points determined, and the complex analyzed through ultraviolet spectroscopy. The spectroscopy data suggest that the protective effect of glucomannan is related to the formation of a supramolecular complex between the two compounds. |
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Keywords: | Aflatoxin B1 Candida utilis comet assay glucomannan supramolecular complex |
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