微米零价铁对剩余活性污泥和餐厨垃圾厌氧联合消化的加强效果及机制

针对剩余活性污泥和餐厨垃圾厌氧联合消化产气效率不高的问题，通过投加微米零价铁，研究其对厌氧联合消化的强化效果及作用机制.结果说明，零价铁的添加强化了厌氧联合消化的产甲烷阶段，但对溶解、水解及酸化阶段没有明显影响.当零价铁的投加量为10 g·L^-1时，经过15 d的厌氧消化，累积甲烷产量（以VS计）达到238.68 mL·g^-1，相比于空白组提高了20.05%.机制分析表明：零价铁提高了系统的电子传递体系（ETS）活性，当零价铁投加量为10 g·L^-1时，最终的ETS活性（以INTF/TS计）达到21.50 mg·（g·h）^-1，而空白组仅为13.43 mg·（g·h）^-1.此外，零价铁还强化了细菌和产甲烷菌之间的直接性种间电子传递（DIET），微生物群落的变化显示DIET相关的微生物，例如Syntrophomonas、Methanosarcina和Methanobacterium，在有零价铁添加的条件下显示出更高的丰度.

Enhancement Effects and Mechanisms of Microscale Zero Valent Iron on the Performance of Anaerobic Co-digestion of Waste Activated Sludge and Food Waste

Focusing on low biogas yields in the anaerobic co-digestion of waste activated sludge and food waste, the enhancing effects and mechanisms of microscale zero valent iron (mZVI) on anaerobic co-digestion was investigated. The results indicated that the addition of mZVI enhanced the methanogenesis stage of co-digestion but had no significant effect on the solubilization, hydrolysis, and acidification stages. With a dosage of 10 g·L^-1 mZVI, the cumulative methane yield (based on VS) within 15 days reached 238.68 mL·g^-1, which was 20.05% higher than the control group. The mechanism analysis showed that mZVI promoted electron transport system (ETS) activity (based on INTF/TS), which increased to 21.50 mg·(g·h)^-1 with 10 g·L^-1 mZVI compared to 13.43 mg·(g·h)^-1 in the control group. Furthermore, mZVI enhanced direct interspecies electron transfer (DIET) between specific bacteria and methanogens. Microbial community analysis demonstrated that the abundance of DIET-related microorganisms, such as Syntrophomonas, Methanosarcina, and Methanobacterium, was higher in presence of mZVI.