Diversity of free-living nitrogen-fixing microorganisms in wastelands of copper
mine tailings during the process of natural ecological restoration |
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Authors: | Jing Zhan and Qingye Sun |
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Institution: | School of Life Science,Anhui University,Hefei 230039,China |
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Abstract: | Biological nitrogen fixing is an important source of nitrogen input in the natural ecological restoration of mine wastelands. The
diversity of nifH genes in tailings samples under di erent plant communities in Yangshanchong and Tongguanshan wastelands
in Tongling, was analyzed using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) approach. The
nitrogen-fixing microorganism community in the upper layer of tailings of Tongguanshan wasteland discarded in 1980 showed
higher Shannon-Wiener diversity index than that in Yangshanchong wasteland discarded in 1991. The diversity of nifH genes in
Yangshanchong wasteland of copper mine tailings did not display a consistent successional tendency with development of plant
communities during the process of natural ecological restoration. Phylogenetic analysis of 25 sequences of nifH gene fragments
retrieved from the DGGE gels indicated that there were mainly two taxa of free-living nitrogen-fixing microorganisms, Proteobacteria
and Cyanobacteria living in the wastelands investigated, most of which were unique and uncultured. Canonical correspondence analysis
(CCA) based on the relationship between band patterns of DGGE profile and physico-chemical properties of tailings samples showed
that the diversity of nifH genes in di erent tailing samples was mainly a ected by loss of ignition, water content, pH and available Zn
contents of wastelands. The dominant plant species and development period of plant communities by ameliorating pH, reducing the
toxicity of heavy metals, increasing organic matter and water content a ected the diversity and structure of the free-living nitrogenfixing
microorganisms in wastelands of copper mine tailings. |
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Keywords: | nifH nitrogen fixation PCR-DGGE natural ecological restoration copper mine tailings |
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