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Cytogenetic biomonitoring of oral mucosa cells of crack cocaine users |
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| Authors: | Maria das Graças Alonso de Oliveira Jean Nunes dos Santos Patrícia Ramos Cury Victor Hugo Pereira da Silva Nara Rejane Cruz Oliveira Ricardo da Costa Padovani Adriana Marcassa Tucci Daniel Araki Ribeiro |
| Institution: | 1. Department of Propedeutics and Integrated Clinic, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil 2. Department of Oral Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil 3. Department of Periodontics, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil 4. Department of Biosciences, Federal University of Sao Paulo, Av. Ana Costa, 95 Vila Mathias, Santos, Sao Paulo, 11060-001, Brazil 5. Department of Human Movement Sciences, Federal University of Sao Paulo, Santos, Sao Paulo, Brazil 6. Department of Health, Education and Society, Federal University of Sao Paulo, Santos, Sao Paulo, Brazil |
| Abstract: | The aim of the present study was to comparatively evaluate genomic damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated oral mucosa cells from crack cocaine users by micronucleus test. A total of 30 crack cocaine users and 30 health controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek mechanically exfoliated, placed in fixative, and dropped in clean slides, which were checked for the above nuclear phenotypes. The results pointed out significant statistical differences (p?<?0.05) of micronucleated oral mucosa cells from crack cocaine users. Exposure to crack cocaine caused an increase of other nuclear alterations closely related to cytotoxicity such as karyolysis in oral cells as well. In summary, these data indicate that crack cocaine is able to induce chromosomal breakage and cellular death in oral mucosa cells of users. |
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