| 微囊藻毒素在银鲫肠道中的累积及其病理学影响 |
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| 引用本文： | 李莉,雷和花,侯杰,龙萌,苏玉晶,吴宁.微囊藻毒素在银鲫肠道中的累积及其病理学影响[J].生态毒理学报,2014,9(6):1189-1196. |
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| 作者姓名： | 李莉 雷和花 侯杰 龙萌 苏玉晶 吴宁 |
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| 作者单位： | 1. 华中农业大学水产学院农业部淡水生物繁育重点实验室淡水水产健康养殖湖北省协同创新中心,武汉,430070
2. 中国科学院武汉物理与数学研究所,武汉,430071 |
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| 基金项目： | 国家自然科学基金项目(No.31100378；No. 51179134)；中央高校基本科研业务费专项资金资助(No. 2010QC022) |
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| 摘 要： | 银鲫(Carassius auratus),杂食性鱼类,是我国淡水主养品种之一。在富营养化湖泊中,它能以有毒微囊藻为主要食物,导致微囊藻毒素(MCs)在其组织中大量累积。为研究MCs在肠道内累积和代谢特征及其对肠道的毒性影响,分别以50和200μg MC-LReq·kg-1剂量的MCs粗提液(主要含MC-RR和MC-LR)对银鲫进行腹腔注射,并在注射后1、3、12、24、48和168 h后取样。MCs的含量用LC-MS和HPLC进行定性和定量测定,结果发现,高低两剂量组银鲫肠中MCs的含量均在注射后1 h达最大值(分别为2.8和181.4 ng·g-1DW),然后随暴露时间的延长迅速下降。相对于毒素的累积,MCs诱导的银鲫肠组织损伤具滞后性,注射后48 h内,高低两剂量组肠道的病理变化呈时间-剂量依赖性的增长,病理特征表现为肠上皮细胞排列紊乱,甚至出现坏死、溶解和脱落,杯状细胞数目显著增多,微绒毛结构破坏并伴随淋巴细胞浸润。实验结果表明,单次染毒后MCs在鲫肠道中迅速累积后降解,并造成时间-剂量依赖性组织损伤,且低剂量组的损伤是可逆的。
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| 关 键 词： | 微囊藻毒素(MCs) 银鲫 肠道 毒性效应 |
| 收稿时间： | 3/6/2014 12:00:00 AM |
| 修稿时间： | 4/4/2014 12:00:00 AM |
Intestinal Accumulation and Pathological Effects of Microcystins in Crucian Carp (Carassius auratus L.) |
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| Li Li,Lei Hehu,Hou Jie,Long Meng,Su Yujing and Wu Ning.Intestinal Accumulation and Pathological Effects of Microcystins in Crucian Carp (Carassius auratus L.)[J].Asian Journal of Ecotoxicology,2014,9(6):1189-1196. |
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| Authors: | Li Li Lei Hehu Hou Jie Long Meng Su Yujing and Wu Ning |
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| Institution: | 1. Fisheries College of Huazhong Agricultural University; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China
2. Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China;Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China;Fisheries College of Huazhong Agricultural University; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China;Fisheries College of Huazhong Agricultural University; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China;Fisheries College of Huazhong Agricultural University; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China;Fisheries College of Huazhong Agricultural University; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China |
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| Abstract: | |
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| Keywords: | microcystins (MCs) crucian carp intestinal tract toxic effects |
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