Antioxidant responses to benzo[a]pyrene, tributyltin and their mixture in the
spleen of Sebasticus marmoratus |
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Authors: | WU Yu-qiong WANG Chong-gang WANG Yun ZHAO Yang CHEN Yi-xin and ZUO Zheng-hong |
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Institution: | 1. Key Laboratory of the Minutry of Education for Cell Biology and Tumor Cell Engineering,School of Life Sciences, Xiamen University,Xiamen 361005,China;Bioengineering Department,MinBei Vocational and Technical College,Jianyang 354200,China 2. Key Laboratory of the Minutry of Education for Cell Biology and Tumor Cell Engineering,School of Life Sciences, Xiamen University,Xiamen 361005,China |
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Abstract: | It has been repotted that there is an interaction between Benzoa]pyrene (BaP),a widespread carcinogenic polycyclic aromatic hydrocarbon,and tributyltin (TBT),an organometal used as an antifouling biocide.This study was therefore designed to examine the potential in vivo influence of BaP,TBT and their mixture on splenic antioxidant defense systems of Sebastiscus marmoratus.The fish were exposed to water containing euvironmentally relevant concentrations of BaP,TBT and their mixture.Spleens were collected for biochemical analysis after exposure for 7,25,50 d and after recovery for 7,20 d.Cotreatment with BaP and TBT for 7 d potentiated the induction of glutathione pemxidase (GPx) activity by BaP or TBT alone.The cotreatment for 25 and 50 d resulted in inhibition of GPx activity,which was similar to the effect of TBT.Splenic glutathione S-transferase (GST) activities were significantly elevated in S.marmoratus exposed to BaP starting from 7d and remained high up to 25 d.However,no further activity change was found with prolonged exposure.Cotreatment of BaP and TBT primarily inhibited the GST activity,which was similar to the effect of TBT.Cotreatment with BaP and TBT for 25 or 50 d potentiated the depletion of GSH (glutathione) by BaP or TBT alone.MDA (malondialdehyde) contents in spleen of S.marmoratus were not significantly altered compared with the control during the test period.Spleen,as an immune organ,is sensitive to exposure of BaP or TBT.It should have an effective mechanism to counteract oxidative damage.Antioxidative defense systems in spleen of S.marmoratus should be considered as potential biomarkers.Short-term exposure of BaP or TBT could result in induction of antioxidant defense system.A significant decrease of these indices.such as GSH,GST,GPx might indicate more severe contamination. |
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Keywords: | tributyltin benzo[a]pyrene antioxidant defense combined effect Sebastiscus marmoratus Antioxidant pyrene marmoratus spleen mixture severe contamination significant decrease indices result antioxidant defense system biomarkers Antioxidative effective mechanism oxidative damage sensitive immune organ |
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