| 硝化悬浮填料塔中氨氧化细菌群落稳定性特征分析 |
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| 引用本文： | 王峰,夏四清,史妍,傅以钢.硝化悬浮填料塔中氨氧化细菌群落稳定性特征分析[J].环境工程,2006,24(3):10-12. |
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| 作者姓名： | 王峰 夏四清 史妍 傅以钢 |
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| 作者单位： | 同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海,200092 |
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| 基金项目： | 上海市教委"曙光计划"
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高等学校博士学科点专项科研项目 |
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| 摘 要： | 利用PCR-DGGE分析技术,对于化学生物絮凝处理工艺中悬浮填料塔的氨氧化细菌(AOB)群落进行了研究。主要考察了曝气强度和水力停留时间2个运行参数调整的情况下,系统AOB群落结构的变化。生物膜样品直接取自硝化填料塔,多维尺度(MDS)方法用于DGGE电泳图谱的分析,分析结果以二维图形向量的方式显示,结果表明,曝气强度和水力停留时间均对AOB群落有一定的影响,主要的AOB种类在运行过程中基本不变,系统对于运行条件具有良好的稳定性。
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| 关 键 词： | 聚合酶链式反应 浓度梯度凝胶电泳 氨氧化菌(AOB) |
| 收稿时间： | 2005-11-18 |
| 修稿时间： | 2005-11-18 |
STABILITY ANALYSIS OF AOB COMMUNITY IN NITRIFYING SUSPENSION FILLER TOWER |
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| Wang Feng, Xia Siqing, Shi Yan.STABILITY ANALYSIS OF AOB COMMUNITY IN NITRIFYING SUSPENSION FILLER TOWER[J].Environmental Engineering,2006,24(3):10-12. |
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| Authors: | Wang Feng Xia Siqing Shi Yan |
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| Institution: | Wang Feng Xia Siqing Shi Yan et al |
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| Abstract: | It was studied that ammonia-oxidizing bacteria(AOB) community in a nitrifying suspension tower for biochemical flocculation process,using PCR-DGGE technique.The change in systematic AOB community structure was examined under the regulation of aeration strength and hydraulic residence time(RHT).The biofilm samples were taken directly from a nitrifying filler tower.MDS method was used for the analysis of DGGE electrophoretogram,and the results were displayed by 2-D pattern.The results show that the aeration strength and RHT have a certain effect on AOB community.The main AOB strains remain unchanged in the process of running. |
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| Keywords: | polyase chain reaction concentration gradient of gel electrophoresis and AOB |
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