Bacterial diversity in Fez tanneries and Morocco’s Binlamdoune River,using 16S RNA gene based fingerprinting

Bacterial diversity in Fez tanneries and Morocco's Binlamdoune River, using 16S RNA gene based fingerprinting

Tannery wastewater causes serious ecological and sanitary damage. Chemical analysis of water from Binlamdoune River of themedina of Fez was conducted and the results revealed the presence of toxic elements from tanneries and other industrial activities,which strongly a ected water quality. To determine the e ectiveness of bioremediation for depollution, we studied the abundanceand diversity of bacteria residing in these polluted environments. Conducting denaturing gradient gel electrophoresis (PCR-DGGE)of the 16S rDNA area using primers related to bacteria showed a bacterial community belonging to eubacterial groups, that is,Epsilonproteobacteria, Clostridia, Lactobacillales, Bacteroidetes, Gammaproteobacteria, and Alphaproteobacteria. In addition, cloningdisplayed the presence of clones belonging to the Firmicutes group. Moreover, scanning electron microscopy revealed a significantheterogeneity of microorganism forms and structures. These endogenous microbes could have a significant role in the purification ofBinlamdoune River and Fez tannery wastewater.