| 不同冬夏寄主棉蚜种群重复序列引物DNA多态性分析 |
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| 引用本文： | 邹晨辉,杨效文,陈晓峰,李迎霞.不同冬夏寄主棉蚜种群重复序列引物DNA多态性分析[J].应用与环境生物学报,2000,6(6):560-564. |
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| 作者姓名： | 邹晨辉 杨效文 陈晓峰 李迎霞 |
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| 作者单位： | 1. 中国科学院动物研究所农业虫害鼠害综合治理国家重点实验室北京 100080
2. 北京工业大学北京 100022 |
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| 摘 要： | 应用重复序列引物ＰＣＲ技术研究了中国不同冬夏寄主上棉蚜种群的ＤＮＡ多态性，通过筛选出的３种适用于棉蚜的重复序列引物，用它们的扩增结果进行相似性指数Ｉｓ和Ｎｅｉ’ｓ遗传距离Ｄ的计算，并根据遗传距离对所研究的棉蚜种群做聚类分析，结果表明，用遗传距离指标可以将冬夏寄主上的棉蚜分开，并且在不同的冬寄主之间，较适宜越冬的不同寄主上的棉蚜呈现出更大的相似性。在实验数据的分析过程中，还发现遗传距离指标比相似性指
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| 关 键 词： | 棉蚜 重复序列引物 遗传距离 寄主植物 种群 |
| 修稿时间： | 1999年12月9日 |
REPEAT SEQUENCE PRIMERS-PCR STUDY ON THE HOST-RELATED POPULATION DIFFERENTIATION OF COTTON APHID, APHIS GOSSYPII IN CHINA |
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| ZOU Chenhui,Yang Xiaowen,CHEN Xiaofeng,Li Yingxia.REPEAT SEQUENCE PRIMERS-PCR STUDY ON THE HOST-RELATED POPULATION DIFFERENTIATION OF COTTON APHID, APHIS GOSSYPII IN CHINA[J].Chinese Journal of Applied and Environmental Biology,2000,6(6):560-564. |
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| Authors: | ZOU Chenhui Yang Xiaowen CHEN Xiaofeng Li Yingxia |
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| Abstract: | The repeat sequence primers-PCR technique was used to determine the DNA polymorphism of aphid, Aphis gossypii(Glover) collected from different summer and winter hosts (Phamnineae, Chinese prickly ash, Hibiscus, Pomegranate and Cotton) in China. 3 primers were selected and used for cluster analysis based on the data of Nei's genetic distance (D) and similarity index (Is). It was found that aphids on summer hosts could be separeted clearly from those on winter hosts by Nei's genetic distance (D), moreover, aphids on suitable winter hosts were more similar in genetic structure. Fig 4, Tab 5, Ref 13 |
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| Keywords: | Aphis gossypii (Glover) repeat sequence primers PCR genetic distance similarity index host plant |
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