| 嗜吡啶红球菌多氯联苯降解基因的克隆与表达 |
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| 引用本文： | 孙艳,杨秀清,钱世钧.嗜吡啶红球菌多氯联苯降解基因的克隆与表达[J].中国环境科学,2004,24(6):0-0. |
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| 作者姓名： | 孙艳 杨秀清 钱世钧 |
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| 作者单位： | 中国科学院微生物研究所,北京,100080 |
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| 基金项目： | 国家“863”项目(2002AA601170) |
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| 摘 要： | 建立了多氯联苯/联苯降解菌嗜吡啶红球菌 R04 的基因组文库.活性筛选得到 1 个18kb的阳性片断,内含 1个 921个核苷酸的新的开环酶2,3-二羟基联苯双加氧酶基因 bphC2,该基因能够在大肠杆菌中表达,其蛋白分子量约为34kDa.此外,在该基因上游39bp处还发现另外一个 2,3-二羟基联苯双加氧酶基因 bphC1 的部分序列.
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| 关 键 词： | 多氯联苯/联苯 生物降解 2 3-二羟基联苯双加氧酶 基因表达 |
| 文章编号： | 1000-6923(2004)06-0734-04 |
| 收稿时间： | 1900-01-01; |
| 修稿时间： | 2004年3月9日 |
Cloning and expression of polychlorobiphenyl/biphenyl degrading gene from Rhodococcus pyridinovorans |
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| SUN Yan,YANG Xiu-qing,QIAN Shi-jun.Cloning and expression of polychlorobiphenyl/biphenyl degrading gene from Rhodococcus pyridinovorans[J].China Environmental Science,2004,24(6):0-0. |
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| Authors: | SUN Yan YANG Xiu-qing QIAN Shi-jun |
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| Abstract: | A degrading genomic library of polychlorobiphenyl biphenyl in Rhodococcus pyridinovorans R04 was constructed. A 18kb fragment was obtained with active screening, containing a new dihydroxybiphenyl dioxygenase gene (bphC2), which could be expressed in E. coli and its protein molecular weight was about 34kDa. In addition, in 39bp in upstream of the gene, there was another partial sequence of 2,3-dihydroxybiphenyl dioxygenase gene bphC1. |
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| Keywords: | polychlorobiphenyl/biphenyl biodegradation 2 3-dihydroxybiphenyl dioxygenase gene expression |
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