| 用基因枪介导法将水稻几丁质酶基因导入小麦 |
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| 引用本文： | 郝建国,李晶,黄萱,徐子勤,贾敬芬,韩德俊,赵宇玮.用基因枪介导法将水稻几丁质酶基因导入小麦[J].应用与环境生物学报,2004,10(4):421-424. |
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| 作者姓名： | 郝建国 李晶 黄萱 徐子勤 贾敬芬 韩德俊 赵宇玮 |
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| 作者单位： | 1. 西北大学生命科学学院,西安,710069
2. 西北农林科技大学,陕西,杨陵,712100 |
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| 基金项目： | 陕西省科技厅基金项目 (2 0 0 0SM1 5)~~ |
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| 摘 要： | 利用PDS1000／氦气基因枪将水稻几丁质酶基因导入小麦幼胚盾片愈伤组织．基因枪轰击后在含有50mg／L硫酸卡那霉素的培养基上经过多步骤筛选和分化，从5个小麦品种共800多个幼胚中获得了6株绿苗，对此6株绿苗进行了PCR和Southern杂交分析．结果表明，其中4株绿苗为转基因植株，转化率最高可达1．2％．图3表2参17
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| 关 键 词： | 小麦 基因枪 水稻几丁质酶基因 转化 |
| 修稿时间： | 2003年6月30日 |
TRANSFERRING RICE CHITINASE GENE INTO WHEAT VIA MICROPROJECTILE BOMBARDMENT |
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| Abstract: | Rice chitinase gene was transferred into immature embryogenic calli of wheat using PDS1000/He particle delivery system. After bombarding immature embryogenic calli of wheat, these embryos were selected and generated on 50 mg/L kanamycine containing medium. Six green plants were regenerated from resistant calli derived from about 800 bombarded immature embryogenic calli from 5 wheat varieties. PCR and southern analysis of 6 green plants showed that 4 green plants contained the target gene. The transformation frequency was 1.2 % at most. Fig 3, Tab 2, Ref 17 |
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| Keywords: | Wheat(Triticum aestivum L ) microprojectile bombardment rice chitinase gene transformation |
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