反硝化细菌强化潜流湿地的污水处理厂尾水脱氮试验

为了揭示反硝化菌强化潜流湿地的污水处理厂尾水脱氮效果及机理,以砾石、红砖碎块、钢渣、陶粒、土壤为湿地填料,茭白、梭鱼草、黑麦草、红叶石楠为湿地植物,构建了两套湿地系统,其中一套投加菌剂,另一套作为对照组,使用双总体t检验方法分析了投加反硝化细菌B8(Pseudomonas putida)菌液于水平潜流湿地系统的操作与生物强化湿地脱氮程度之间的相互关系。结果表明,将反硝化菌(B8)菌液连续14 d投加于水平潜流湿地后,在强化潜流湿地运行的58 d内,其NH_4~+-N、NO_2~--N和TN平均去除率分别为65.3%、94.2%和71.5%;而未投菌的潜流湿地的NH_4~+-N、NO_2~--N和TN平均去除率分别为28.2%、74.7%和43.1%,加入菌剂使潜流湿地氮素去除能力大幅提高。双总体t检验方法分析表明,在停止投菌运行的41 d内,接种B8细菌的湿地系统的总氮去除率显著高于未投菌的湿地系统(p0.05);但在停止投菌运行的58 d内,投菌湿地和未投菌湿地脱氮效果的差异不显著(p0.05),因此确定B8强化水平潜流湿地系统的投菌周期为58 d。

Experiment for enhancing nitrogen removal from the effluent sewage treating plant via the denitrifying bacteria in the context of subsurface flow constructed wetland

The paper is inclined to present the results of its experimental study for enhancing the nitrogen removal from the effluent sewage treating plant via the denitrifying bacteria in the context of subsurface flow constructed wetland.For this purpose,we have analyzed the correlation between the dosing microbes and the bio-augment function of the denitrifying bacteria B8 (Pseudomonas putida) in such wetlands through one-to-one comparing method.It is just for this purpose that we have established two sets of wetland systems (one of the systems is added by B8 strain agent,while the other is the control test group intended with no B8 strain agent) with gravels,red brick pieces,iron-steel slag,ceramsite and soil as the substrate media and Zizania aquatic,Pontederia cordata,Lolium perenne,Photiniaxfraseri as the macrophyte.In so doing,we have also cultured B8 strain and the horizontal subsurface flow constructed wetland as influent for 14 consecutive days with the tail-water and the condition of self-inoculation.The results of our contrast studies indicate that on the 58th day,it would be possible to make the mean removal rates of the ammonia nitrogen,nitrite and total nitrogen (TN) increase to 65.3％,94.2％ and 71.5％,respectively,via inoculation,though in the control testing group under the same testing environment the removal rates remain merely 28.2％,74.7％ and 43.1％ with no addition of the strain agent.Thus,it can be seen that adding the strain agent can greatly heighten the nitrogen removal ability through inoculation.What is more,through the contrast analysis of the results of the pair-matched experimental tests,it has been found that the total nitrogen removal efficiency of the wetland system can be greatly promoted by adding B8 strain agent as a result of 41 days (p ＜ 0.05) comparative tests.Nevertheless,no obvious difference has been noticed between the wetland system added by B8 strain agent and the control test group as a result of the testing process of 58 days (p ＞ 0.05),which indicates that the best adding B8 bacteria cycle of subsurface flow constructed wetland should last 58 days.