氨基比林在氯化消毒前后对小球藻生长及生理特性的影响

为探讨典型的吡唑酮类药物——氨基比林(Aminopyrine,AMP)在氯化消毒前后对普通小球藻生长及生理特性的影响,设置了生长抑制试验和生理特性影响试验。在生长抑制试验中,设定药物暴露质量浓度为0.1~32.0 mg/L,共7个处理组,以不加药物组为对照组,再按照药物与氯气(C12)物质的量比为4:1、1:1、1:2、1:5分别向各处理组加入相应的NaClO溶液,并氮吹至余氯在检测限下,测定小球藻的生物量。结果表明:72 h内小球藻的生长抑制率先升高后降低,并随药物质量浓度升高而升高;氯化消毒处理后,抑制率随投氯量增加而上升。在生理特性影响试验中,设置药物质量浓度为2.0~32.0 mg/L,共5个处理组,其他处理同生长抑制试验,测定培养96 h后的抗氧化酶(超氧化物歧化酶SOD、过氧化氢酶CAT)活性和丙二醛(MDA)含量,结果表明:MDA含量随机体抗氧化酶活性降低而升高;氯化消毒处理后,1:5处理组的SOD和CAT活性均低于氯化消毒前,而MDA含量均高于氯化消毒前,表明该比例的氯化消毒对小球藻的抑制作用较其他各组更为显著。

Influence of aminopyrine on the growth of Chlorella vulgaris and analysis of its physiological features with and without chlorination

The article is aimed at reporting the study results of the influence of aminopyrine on the growth of the Chlorella vulgaris and an analysis of its physiological features with and/or without chlorination.As is known,chlorine is one of the commonest disinfectants for sewage and drinking water treatment,though it is unfortunate that there has been found disinfective by-products as a result of free chlorine disinfection in recent years.One of the typical pyrazolone compounds,i.e.the aminopyrine (AMP) that has frequently been found in the aquatic environments,which can often been chosen for exploring its potential eco-toxicity with and without chlorination.It is just for finding the potential eco-toxicity of aminopyrine that we have laid out a series of growth inhibition and physiological effect tests by using Chlorella vulgaris.The eventual aim is to find the inhibition rate of the average growth and the changes of the physiological characteristics of the Chlorella vulgaris.Therefore,we have laid out seven treating groups with their initial concentrations of AMP being from 0 (control) to 32.0 mg/L.in correspondence of NaCl0 solution added to each treating group of chlorine (Cl2) in different molar concentration ratios,such as four to one;one to one,one to two and one to five,respectively,so as to test the removal rate of the residual chlorine in the solution till the residual chlorine wouldbe remaining below the detective limit via the nitrogen-blowing method.All treatments should be done thrice under the similar conditions as has been shown above with their average data given.And,the results of the said growth-inhibiting tests have finally proven that the growth inhibition rate of the Chlorella vulgaris tends to increase at first and then decrease at the same drug concentration within 72 hours.After chlorination treatment,the inhibition rate may turn to increase with the increase of the chlorine dosage.On the other hand,in the test for the physiological features,we have tested and measured the activity of the antioxidant enzymes (superoxide dismutase,i.e.SOD,catalase,i.e.CAT) and the malondialdehyde (MDA) content as a result of 96 hours' culture.The testing results indicate that the MDA content tends to rise with the decrease of the antioxidant enzyme activity.What is more,the SOD and CAT in 1:5 treatment groups tend to drop whereas the content of MDA turns to increase after the chlorination treatment,thus showing that it would be more effective to take greater chlorine dosage in hoping to inhibit the Chlorella vulgaris.All in all,all the above experiment results can provide some valuable technical references for the application of chlorination technology in drinking water treatment and some useful theoretical grounds for human health protection.