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Monitoring of the Degradation Activities and the Diversity of the Microbial Community Degrading Refinery Waste Sludge |
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| Authors: | Katsivela Eleftheria Moore Edward R. B. Kalogerakis Nicolas |
| Affiliation: | 1. Laboratory of Biochemical Engineering & Environmental Biotechnology, Department of Environmental Engineering, Technical University of Crete, Polytechneioupolis, GR-73100, Chania, Greece 2. Department of Natural Resources and Environment, Technological Educational Institute of Crete, Romanou 3, Halepa, GR-73133, Chania, Crete, Greece 3. The Soil Health Initiative, Department of Soil Quality and Protection, Macaulay Land Use Research Institute, AB15 8QH, Aberdeen, Scotland, U.K 4. Division of Microbiology, National Research Centre for Biotechnology (GBF), Mascheroder Weg 1, D-38124, Braunschweig, Germany |
| Abstract: | The biodegradation of n-alkanes and branched alkanes from waste sludge were observed in landfarming soils of Motor Oil Hellas (a petroleum refinery) and changes in the bacterial communities in the soils were monitored during the remediation. Bacterial 16S rRNA gene (rDNA)-based community fingerprint patterns were obtained from soil samples by terminal restriction fragment length polymorphism (T-RFLP) analysis. Changes in T-RFLP fingerprints, as well as in the petroleum contaminant composition of the polluted soil, correlated with degradation activities in field tests. |
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