渗滤液耦合矿化垃圾制备甲烷氧化菌菌液

利用甲烷氧化菌菌液增加材料中甲烷氧化菌的数量,可以得到高甲烷氧化率的填埋场甲烷生物氧化覆盖材料。研究发现,渗滤液原水和渗滤液处理尾水均能促进甲烷氧化菌的生长,可利用渗滤液耦合矿化垃圾混合培养制备甲烷氧化菌菌液用于填埋场甲烷减排。填埋龄长的渗滤液原水有着较好的培养效果,得到的菌液在4 d内最高甲烷氧化速率达到2.68 mL/h,超过甲烷氧化菌培养液(nitrate minimal salt medium,NMS)的实验结果。渗滤液中总氮、无机碳、总有机碳和Ni元素的含量对甲烷氧化菌的培养过程影响较大,适用于甲烷氧化培养的渗滤液应满足:总氮1 400 mg/L,总有机碳55 mg/L,Ni元素0.4 mg/L,总磷含量较高。

Mixed-cultivation of methanotrophs by using leachate coupled with aged refuse

Methanotrophs agent could communicate methane oxidation capacity of cover materials obviously by increasing methanotrophic organisms in materials. It was found that leachate could be used as substrate coupled with aged refuse for methanotrophs mix-cultivation instead of nitrate minimal salt medium (NMS), and the peak methane oxidation rate of methanotrophs liquid was 2.68 mL/h on the 4th day measured for older untreated leachate, which was higher than that for NMS. Contents of TN, IC, TOC and Ni in leachate had most influence on methanotrophs cultivation. The suggested physicochemical properties of leachate used for methanotrophs mix-cultivation were found to be TN<1 400 mg/L, TOC>55 mg/L, Ni<0.4 mg/L, and higher TP value.