荧光原位杂交技术解析发酵产氢细菌群落结构

应用荧光原位杂交(FISH)技术,研究了连续流搅拌槽式(CSTR)发酵制氢反应器中2种产氢发酵类型的菌群组成和发酵类型转化过程中的菌群种类和数量变化及其对产氢速率、生物量和发酵产物的影响.结果表明,梭菌属和肠杆菌科在决定产氢发酵类型方面有重要作用.以梭菌为优势种群的乙醇型发酵比以肠杆菌为优势种群的丁酸型发酵具有更佳的产氢能力.实验结果能够与生物发酵系统常规监测指标形成很好的印证.

Community structures of fermentative hydrogen-producing bacteria community by fluorescent in situ hybridization

Applying fluorescent in situ hybridization (FISH) technique, 2 kinds of community composition of hydrogen production, fermentative type and bacteria community kinds and amount variance and its influence on hydrogen production rate, biological quantity and fermentation product in the transform process of fermentation type in the continuous stir tough reactor (CSTR) fermentation hydrogen production reactor were studied. Clostridium and Enterobacteriaceae had important action on deciding the hydrogen production fermention type; the ethanol type fermentation using Clostridium as the dominant group possessed the best hydrogen producing ability compared with the butyric acid type fermentation using Enterobacteriaceae as the dominate group. The test results could form very good corroborating with conventional monitor index of biofermentation system.