DMF降解菌MBYD-1的分离、鉴定及降解特性

从农药厂污水处理池的活性污泥中分离得到一株能以二甲基甲酰胺(DMF)为唯一碳、氮源生长的菌株MBYD-1.经形态观察、生理生化实验及16S rRNA基因序列分析,将菌株初步鉴定为假单胞菌属 (Pseudomonas sp.).菌株MBYD-1降解DMF的最适条件为:pH 7.0,30℃.当DMF浓度为400mg/L,接种量为2%时,菌株能在72h内将DMF完全矿化.底物广谱性实验表明,菌株MBYD-1能在甲酸盐和甲醛中很好地生长,在甲酰胺中的生长情况优于二甲胺.

Isolation, identification and degradation characteristics of a DMF-degrading bacterial MBYD-1

A bacterial strain MBYD-1, capable of utilizing DMF (N, N-dimethylformamide) as sole carbon and nitrogen source, was isolated from activated sludge collected from wastewater-treating system of a pesticide manufacturer. This strain was identified as Pseudomonas sp. according to its morphological, physiological and biochemical analysis and the analysis of its 16S rRNA gene sequence, and named strain MBYD-1. The optimal pH and temperature for the degradation were 7.0 and 30℃, respectively. Strain could mineralize DMF completely in 72 hours when the initial concentration of DMF was 400 mg/L, and with 2% inoculating quantity. Degradable substrates test showed that strain MBYD-1 grew better in formate than formaldehyde, and grew better in formamide than dimethylamine.