| 天然水华蓝藻中微囊藻毒素的提取和净化研究 |
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| 引用本文： | 张维昊,肖邦定,方涛,张锡辉,宋立荣.天然水华蓝藻中微囊藻毒素的提取和净化研究[J].环境污染与防治,2003,25(5):265-267. |
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| 作者姓名： | 张维昊 肖邦定 方涛 张锡辉 宋立荣 |
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| 作者单位： | 1. 清华大学深圳研究生院环境工程和管理研究中心,深圳,518057
2. 中国科学院水生生物研究所,武汉,430071 |
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| 基金项目： | 国家 8 63项目资助 (2 0 0 1AA64 10 3 0 )，国家自然科学基金重点项目 (No.5 0 2 3 80 2 0 ) |
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| 摘 要： | 利用天然水华蓝藻为原料，通过筛选、比较不同极性提取剂对微囊藻毒素的提取效果，80％甲醇溶液有较高的提取效率，而乙醇溶液可实现无毒提取，优化了提取方法。并通过调节溶液pH为藻胆蛋白等电点的方法去除共提取的大量藻胆蛋白，取得了很好的净化效果，为微囊藻毒素的分离和纯化研究提供了基础。
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| 关 键 词： | 天然水华蓝藻 微囊藻毒素 提取 净化 水体污染 |
Extraction and purification of microcystins from natural cyanobacterial bloom |
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| Zhang Weihao,et al..Extraction and purification of microcystins from natural cyanobacterial bloom[J].Environmental Pollution & Control,2003,25(5):265-267. |
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| Authors: | Zhang Weihao |
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| Institution: | Zhang Weihao,et al. Research Center for Environmental Engineering & Management Shenzhen Graduate School of Tsinghua University,Shenzhen 518057 |
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| Abstract: | The extraction and purification of microcystin from natural cyanobacterial bloom were studied. The optimized method was assessed with dfferent solvents applied to assess the efficiency for microcystin extraction. 80% methanol has highest efficiency and ethanol solvent could extract microcystins safely. The massive of phycobiliprotein was removed by adjusting the pH of solvents to the isoelectric point. This result provides a solid basis for obtaining pure microcystins efficiently. |
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| Keywords: | Natural bloom\ Cyanobacterial powder\ Microcystins\ Extract\ Purify |
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