The effects of elevated CO2 on clonal growth and nutrient content of submerge plant Vallisneria spinulosa

An approximately four months long glasshouse experiment was conducted to examine the effects of elevated carbon dioxide (CO(2)) concentration (1,000 +/- 50 micromol mol(-1)) in the atmosphere on biomass accumulation and allocation pattern, clonal growth and nitrogen (N), phosphorus (P) accumulation by the submerged plant Vallisneria spinulosa Yan. Elevated CO(2) significantly increased V. spinulosa total fresh biomass ( approximately 130%) after 120 days, due to more biomass accumulation in all morphological organs than in those at ambient CO(2) (390 +/- 20 micromol mol(-1)). About 75% of the additional total biomass at elevated CO(2) was accounted for by leaf and rhizome (above ground) biomass and only 25% of it belonged to root and turion (below ground). However, the turions biomass exhibited a greater increase rate than that of organ above ground, which caused reduction in the above/below ground biomass ratio. The clonal growth of V. spinulosa responded positively to elevated CO(2). The number of primary ramets increased up to 1.4-folds at elevated CO(2) and induced a dense growth pattern. For nutrients absorption, concentration of N in leaf and in turion was significantly (p