壬基酚和辛基酚暴露的生物标志物——尿液5-HT

为了探讨壬基酚和辛基酚对大鼠体内(5-HT)代谢的影响,为壬基酚和辛基酚暴露标志物的鉴定提供依据.选取健康雄性Sprague Dawley大鼠35只,随机分为对照组,壬基酚、辛基酚及其联合染毒低剂量组(50 mg·kg-1·d-1),壬基酚、辛基酚及其联合染毒高剂量组(150 mg·kg-1·d-1),每组5只,连续7d灌胃染毒,建立染毒动物模型.灌胃次日,利用高效液相色谱法(HPLC)检测各组大鼠尿液中5-羟色胺(5-HT)含量.结果表明,染毒组大鼠尿液中5-HT浓度均显著高于正常对照组(p＜0.01)；高剂量染毒组大鼠尿液中5-HT浓度均显著高于其低剂量染毒组(p＜0.01)；染毒第7天大鼠尿液中5-HT浓度均显著高于其染毒第3天(p＜0 01)尿液中5-HT浓度随染毒剂量和染毒时间的增加而升高,有剂量-毒性效应和时间-毒性效应关系；尿液5-HT可作为评估壬基酚和辛基酚暴露的潜在生物标志物,应用于大规模人群暴露风险的监测.

Identification of urine 5-hydroxytryptamine as the biomarker of nonylphenol and octylphenol exposure

This study aims to investigate the effects of nonylphenol (NP) and ocytlphenol (OP) on the metabolism of 5-hydroxytryptamine (5-HT) and to provide the basis for the biomarker identification of NP and OP exposure. The 40 male SD rats were randomly selected and divided into seven groups including control group, low-dose NP/OP/combination group (50 mg·kg^-1·d^-1) and high-dose NP/OP/combination group (150 mg·kg^-1·d^-1), with 5 rats in each group.The concentrations of urine 5-HT in SD rats were detected by high performance liquid chromatography. The concentrations of urine 5-HT in exposed rats are significantly higher than the control group (p< 0.01). Compared with low dose groups, the content of urine 5-HT in high dose exposed rats increases (p< 0.01). Significant increase of urine 5-HT concentration was found in exposed rats on the 7^th day than that on the 3^rd day (p< 0.01). Effects of dose-toxicity and time-toxicity were found between the concentration of urine 5-HT and the exposure to NP and OP. Urine 5-HT could be used as a tool to evaluate the contamination of NP and OP and predict the exposure risk for a large-scale population.