菌藻复合体系氮代谢性能及菌群特征

为了城市尾水深度脱氮，控制地表水体富营养化，利用分子生物学等方法，分别对脱氮复合菌群和藻菌共生系统的氮代谢性能进行研究.结果表明，氮代谢复合菌群具有较高氨化和反硝化性能.JZ组对城市尾水中氨氮的去除效果最好，降解率高达95%以上，在实验室模拟污水中JZ组系统总氮去除效果优于J组.高通量测序显示，J组主要优势菌群及占比分别为：厚壁菌门44.53%，变形菌门43.41%，放线菌门5.37%，类杆菌门3.04%，绿弯菌门1.35%；JZ组丰度主要优势菌群和占比分别为：蓝藻门33.89%，绿弯菌门25.34%，变形菌门19.38%，厚壁菌门10.02%，酸杆菌门4.20%；各优势物种在J组和JZ组比例不同，厚壁菌门分别为82%和18%，变形菌门为69%和31%，蓝藻门为1%和99%，绿弯菌门为5.1%和95%，放线菌门分别为73%和27%.表明JZ组在城市尾水中去除氨氮效果较好，J组氮代谢菌群随着微球藻加入和生长，JZ组氮代谢菌群构成随之发生变化，以适应优势藻类形成的环境，与微球藻构成新的菌藻氮代谢体系.研究结果为藻菌共代谢体系应用提供了理论和数据基础.

Nitrogen Metabolism and Flora Characteristics of Bacteria Algae Complex System

In order to denitrify the urban tail water deeply and control the eutrophication of surface water, the molecular biology methods were used to study the nitrogen metabolism performance of the denitrification complex flora and the algal-bacteria symbiotic system. The results showed that the nitrogen metabolism complex flora was high ammonification and denitrification performance. The removal effect of ammonia nitrogen of group JZ was very well in urban tailwater, and the degradation rate was as high as 95%. The removal effect of total nitrogen of group JZ was better than that of group J in the experimental water distribution. High-throughput sequencing showed that the main dominant flora and proportion of group J were Firmicutes 44.53%, Proteobacteria 43.41%, Actinobacteria 5.37%, Bacteroidetes 3.04%, and Chloroflexi 1.35%. The main dominant bacterial groups in the group JZ were 33.89% Cyanobacteria, 25.34% Chloroflexi, 19.38% Proteobacteria, 10.02% Firmicutes, and 4.20% Acidobacteria. The dominant species in group J were compared with those in group JZ; the proportions were 82% and 18% in Firmicutes, 69% and 31% in Proteobacteria, 1% and 99% in Cyanobacteria, 5.1% and 95% in Chloroflexi, 73% and 27% in Actinobacteria. It was concluded that the removal effect of ammonia nitrogen of group JZ was high in the urban tailwater. With the addition and growth of Micrococcus in group J, the nitrogen metabolism flora in group JZ changed accordingly, so as to adapt to the environment in which the dominant algae formed. It forms a new nitrogen metabolism system of bacteria and algae with Micrococcus. This research provides a theoretical and data basis for the application of algal-bacterial co-metabolism systems.