| 用于石油污染土壤降解的高效降解菌的筛选及其降解条件优化 |
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| 引用本文： | 武海杰,张秀霞,白雪晶,郭云霞.用于石油污染土壤降解的高效降解菌的筛选及其降解条件优化[J].环境工程学报,2014,8(3):1229-1234. |
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| 作者姓名： | 武海杰 张秀霞 白雪晶 郭云霞 |
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| 作者单位： | 中国石油大学环境与安全工程系, 青岛 266580;中国石油大学环境与安全工程系, 青岛 266580;中国石油大学环境与安全工程系, 青岛 266580;中国石油大学环境与安全工程系, 青岛 266580 |
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| 基金项目： | 中国石油科技创新基金（2009D-5006-07-01）；中央高校基本科研业务费专项基金（27R1104052A）；青岛市科技计划项目（KJZD-12-65-jch）；中国石油大学（华东）研究生创新工程（CX-1219，CX2013035） |
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| 摘 要： | 经过富集、分离优选出高效石油降解菌L-1,根据形态观察和生理生化特征初步鉴定为琼氏不动杆菌;采用单因素花盆实验模拟微生物原位修复并对其降解条件进行优化。结果表明,将高效石油降解菌应用于修复石油污染土壤,适宜接种量、表面活性剂浓度、CNP比、翻耕频率分别为15%、0.1%、100∶10∶1和1 d 1次;在该降解条件下修复28 d,可达到16.80%的石油降解率,远远高于土著微生物6.92%的降解率。
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| 关 键 词： | 石油降解菌 筛选 降解条件 土壤修复 |
Isolation and optimization degradation conditions of highly efficient degrading bacteria for oil-contaminated soil |
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| Wu Haijie,Zhang Xiuxi,Bai Xuejing and Guo Yunxia.Isolation and optimization degradation conditions of highly efficient degrading bacteria for oil-contaminated soil[J].Techniques and Equipment for Environmental Pollution Control,2014,8(3):1229-1234. |
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| Authors: | Wu Haijie Zhang Xiuxi Bai Xuejing and Guo Yunxia |
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| Institution: | Department of Environmental and Safety Engineering, China University of Petroleum, Qingdao 266580, China;Department of Environmental and Safety Engineering, China University of Petroleum, Qingdao 266580, China;Department of Environmental and Safety Engineering, China University of Petroleum, Qingdao 266580, China;Department of Environmental and Safety Engineering, China University of Petroleum, Qingdao 266580, China |
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| Abstract: | Highly efficient petroleum-degrading bacteria L-1 was isolated by enrichment and separation,and was preliminarily identified as Acinetobacter junii by morphology observation,and its physiological and biochemical characteristics. Single factor flowerpot experiments were used to simulate microorganism in-situ remediation and optimize its degradation conditions. Results showed that highly efficient petroleum-degrading bacteria was applied to oil-contaminated soil,the optimum inoculation amount,content of surfactant,ratio of CNP,plowing frequency were 15%,0.1%,100:10:1 and once a day,respectively. Remedying 28 days under these conditions,oil degradation rate reached 16.80%, much higher than 6.92% of indigenous microorganism. |
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| Keywords: | petroleum-degrading bacteria isolation degradation condition soil remediation |
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