| 高效降解微囊藻毒素食酸戴尔福特菌USTB-04的培养与活性研究 |
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| 引用本文： | 徐春霞,何宏胜,张超,吕乐,闫海.高效降解微囊藻毒素食酸戴尔福特菌USTB-04的培养与活性研究[J].环境工程学报,2007,1(5):21-24. |
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| 作者姓名： | 徐春霞 何宏胜 张超 吕乐 闫海 |
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| 作者单位： | 1. 河北农业大学海洋学院,秦皇岛,066003
2. 北京科技大学应用科学学院生物科学与技术系,北京,100083 |
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| 基金项目： | 国家自然科学基金
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北京市自然科学基金
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教育部科学技术研究项目 |
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| 摘 要： | 主要针对筛选的高效降解微囊藻毒素(microcystins,MCs)的食酸戴尔福特菌USTB-04(Delftia acidovorans,DA菌)的培养方法进行了研究.结果表明,以葡萄糖、甘油和乙醇作为惟一碳源时,与氯化铵和尿素相比,酵母粉是支持DA菌生长的较好氮源.在以酵母粉作为惟一氮源时,与甘油和乙醇相比,葡萄糖是提高DA菌生长速度的较好碳源.进一步研究显示,以葡萄糖和酵母粉作为碳源和氮源时,可以支持DA菌的快速稳定生长,但在以甘油和酵母粉作为碳源和氮源时.培养出的DA菌降解MCs的比活性最高.此研究在培养高细胞浓度DA菌作为生物催化剂用于饮用水源中的MCs去除方面具有重要意义.
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| 关 键 词： | 微囊藻毒素 食酸戴尔福特菌 USTB-04 生物降解 培养 活性 高效降解 微囊藻毒素 食酸戴尔福特菌 培养方法 活性研究 microcystins biodegradation efficient activity 意义 饮用水源 生物催化剂 高细胞浓度 比活性 稳定 快速 显示 生长速度 支持 酵母粉 |
| 文章编号： | 1673-9108（2007）05-0021-04 |
| 收稿时间： | 9/6/2006 12:00:00 AM |
| 修稿时间： | 2006-09-062007-01-23 |
Culture and activity of bacterical Delftia acidovorans USTB-04 for efficient biodegradation of microcystins |
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| Xu Chunxi,He Hongsheng,Zhang Chao,Lu Le and Yan Hai.Culture and activity of bacterical Delftia acidovorans USTB-04 for efficient biodegradation of microcystins[J].Techniques and Equipment for Environmental Pollution Control,2007,1(5):21-24. |
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| Authors: | Xu Chunxi He Hongsheng Zhang Chao Lu Le and Yan Hai |
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| Institution: | Ocean College, Agriculture University of Hebei, Qinhuangdao 066003,Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083,Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083,Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083 and Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083 |
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| Abstract: | The culture of an isolated Delftia acidovorans USTB-04 for the biodegradation of microcystins (MCs) was investigated. Compared with ammonium chloride and urea, yeast extracts was found to be a better nitrogen source for the growth of D.acidovorans USTB-04 when glucose, glycerol and ethanol were used as carbon sources, respectively. It was also indicated that glucose was better carbon source compared with glycerol and ethanol for the growth of D.acidovorans USTB-04 when yeast extract was used as a sole nitrogen source. Further studies showed that glucose and yeast extract supported the rapid growth of D.acidovorans USTB-04, however D.acidovorans USTB-04 grown on glycerol and yeast extracts maintained the high biodegradation activity of MCs, which has great significance in the removal of MCs from drinking water supplies using D.acidovorans USTB-04 as a kind of bio-catalyzer. |
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| Keywords: | microcystins Delfiia acidovorans USTB-04 biodegradation culture activity |
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