生物强化对MBR系统生物特性及群落结构的影响

采用高效菌强化膜生物反应器对溴氨酸废水进行处理,考察了生物强化前后系统对溴氨酸的降解能力及其内部微生物生理状态变化.并采用核糖体基因间隔序列分析技术(RISA)解析了高效菌投加前后污泥系统的群落变化.实验表明,投菌后在进水负荷增加的条件下,上清液和膜出水的溴氨酸脱色率分别约为50%和65%,相应的COD去除率分别约为25%和50%,和投菌前基本保持一致;TTC-脱氢酶活性和胞外聚合物(EPS)浓度略有波动,但是运行一段时间后即恢复到投菌前水平.群落分析表明高效菌可以在系统中稳定存在,并且不对原菌群结构造成较大影响.

Microbial characteristics and community analysis of bioaugmented MBR system

The membrane bioreactor (MBR) bioaugmented by a strain with high degradability for bromamine acid was used for treating bromamine acid wastewater. The biodegradation ability and the physiological state changes were studied before and after the high efficiency strain was added. And the ribosomal intergenic sequence analysis (RISA) was used to show the changes of microbial community during the period. The results show that after inoculation, the decolorization rates of the supernatant and the membrane effluent remained 50% and 65% approximately, and the removal rates of COD were about 25% and 50%, respectively. Although these results were similar to that of the system without high efficiency strain, however the influent load was increased, thus the absolute removal amount was increased. The TTC-dehydrogenase activity and the extracellular polymeric substances (EPS) fluctuated slightly after inoculation, and then were back to the earlier level after a period of operation. Microbial community analysis shows that the inoculated high efficiency strain could steadily survive in the system and did not affect the indigenous populations of the microbial communities.