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| 11个猕猴桃品种间的遗传多样性分析 | |
| 引用本文： | 邹游,丁建,申瑛,赵建,杨志荣,吴成.11个猕猴桃品种间的遗传多样性分析[J].应用与环境生物学报,2007,13(2):172-175. |
| 作者姓名： | 邹游 丁建 申瑛 赵建 杨志荣 吴成 |
| 作者单位： | 1. 四川大学生命科学学院,成都,610064 2. 四川省自然资源研究所猕猴桃研究开发中心,成都,610015 3. 四川申能科技有限责任公司,成都,610036 |
| 摘 要： | 利用随机扩增多态性DNA标记(RAPD)技术对采自四川省猕猴桃科研基地的11个猕猴桃品种进行了遗传多样性分析.通过引物筛选,从15个RAPD引物中扩增出135条带,其中100条为多态带,占74.07%.UPGMA聚类分析结果揭示了各品种间的亲缘关系.RAPD标记说明猕猴桃品种间遗传距离与地理分布有一定关系,地理位置较近的材料能聚在一起.分子标记可用于猕猴桃种质资源的分类、鉴定及良种选育.图2表2参15 |
| 关 键 词： | 猕猴桃 遗传多样性 |
| 收稿时间： | 2006-06-22 |
| 修稿时间： | 2006-11-10 |
Analysis of Random Amplified Polymorphic DNA in 11 Actinidia Varieties |
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| ZOU You,DING Jian,SHEN Ying,ZHAO Jian,YANG Zhirong,WU Cheng.Analysis of Random Amplified Polymorphic DNA in 11 Actinidia Varieties[J].Chinese Journal of Applied and Environmental Biology,2007,13(2):172-175. | |
| Authors: | ZOU You DING Jian SHEN Ying ZHAO Jian YANG Zhirong WU Cheng |
| Institution: | 1. College of Life Sciences, Siehuan University, Chengdu 610064, China;2.Centre of Kiwifruit Research and Development, Sichuan Natural Resources Research Institution, Chengdu 610015, China;3.Sichuan Shen Neng Technology Co. Ltd. , Chengdu 610036, China |
| Abstract: | Random amplified polymorphic DNA(RAPD)was applied to detect genetic diversity of the samples of 11 Actinidia varieties collected from the Centre of Kiwifruit Research and Development,Sichuan Natural Resources Research Institution.15 RAPD primers produced 135 bands,of which 100 bands were polymorphic.The polymorphic bands accounted for 74.07% of the total bands.UPGMA(Un-weighted Pair Group Mathematics Average)clustering analysis displayed the genetic relation among the varieties.The RAPD markers indicated that the genetic distance of Actinidia varieties was related with their distribution,and some of them distributing closely could get together.Molecular markers could be used for classifying and identifying the germplasms of Actinidia,as well as for breeding of fine varieties.Fig 2,Tab 2,Ref 15 |
| Keywords: | RAPD |
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