微生物-生物促生剂协同修复河道底泥——促生剂投量对修复效果的影响

采用5个110L的模拟河道反应器，在投加菌剂的基础上（底泥稳定后向底泥和水中分别注射浓度为0.09%和0.03%的菌剂），生物促生剂投量分别设计为0.08，0.09，0.10和0.11g/L，单独投加菌剂组作为空白实验.通过为期四十余天的实验研究微生物与促生剂协同作用时的修复效果.结果表明，促生剂投量为0.10g/L时，上覆水COD去除率69%高于其余各组；各协同修复组上覆水中NO₃^--N基本无积累，且促生剂投量为0.11g/L时NO₃^--N去除率最高，可达96%；40d内各组底泥TOC，TN含量变化不显著，延长反应时间至84d，各协同修复组底泥TOC，TN去除率均有明显提升.各协同修复组脲酶活性均波动下降，蛋白酶活性在15d左右时达到峰值，增幅可达106%~237%.通过高通量测序技术发现，43d时Desulfuromonas，Pseudomonas，Treponema_2，Blvii28_wastewater-sludge_group等主要功能菌群的相对丰度与空白实验组相比均增加，且促生剂投量为0.10g/L可使微生物群落向更适宜降解去除氮素及有机质的方向演替，有效改善底泥环境.协同修复对底泥，上覆水水质及微生物群落有良好改善效果，且促生剂投量为0.1g/L时整体修复情况更为理想.

Microorganism and biostimulant collaboratively remediate river sediment-Influence of biostimulant quantity on repair performance

By using five 110L simulated river reactors, the biostimulant dosage was designed as 0.08, 0.09, 0.10 and 0.11g/L, respectively, with adding aerobic denitrifiers (sediment and water were injected at a concentration of 0.09% and 0.03% of the agent), and the aerobic denitrifiers supplement group was used as a blank experiment, to study the repair effect in the collaborative effect of microorganism and biostimulant through more than 40days' experiment. The results showed that when the dosage of biostimulant was 0.10g/L, and the removal rate of COD in overlying water was 69%, which was higher than that of the other groups. NO₃^--N in the overlying water of each synergistic remediation group had no accumulation. The NO₃^--N removal rate is the highest at up to 0.11g/L, which is up to 96%; the TOC and TN content of sediments in each group did not change significantly within 40days. The TOC and TN removal rates of the sediment were significantly improved when the reaction time was extended to 84days. The urease activity of each collaborative group fluctuated, and the protease activity peaked at around 15d, with an increase of 106%~237%. Through high-throughput sequencing technology, the relative abundance of major functional groups, such as Desulfuromonas, Pseudomonas, Treponema_2, and Blvii28_wastewater-sludge_group increased at 43^rd day compared with the control group alone, and the biostimulant dosage was 0.10g/L can enable the microbial community to be more suitable for the degradation of nitrogen and organic matter in the direction of succession, effectively improving the sediment environment.