菌糠强化微生物降解石油污染土壤修复研究

采用菌糠协同高效石油烃降解菌Microbacterium.sp.Q2进行石油污染土壤修复试验研究，分别设置菌糠固定化微生物组（SIM）、菌糠-游离菌组（SMSB）、菌糠单独组（SMS）和对照组（CK）4组修复实验.考察不同处理方式下对石油污染土壤微生物数量、酶活性和石油烃降解效果的差异性并确定石油污染土壤的最佳修复方案.结果表明：不同修复方式下，SIM组的土壤呼吸强度、微生物数量及酶活性较其他组有明显提高，其对石油烃去除率分别比其他3组提高11.84%、22.15%、54.09%.土壤中脱氢酶活性以及微生物活性与石油烃降解率的相关性显著，此外菌糠固定化微生物对石油污染土壤修复具有生物强化和生物刺激协同的作用机制.

Bioremediation of petroleum contaminated soil by microoganisms enhanced with spent mushroom substrate

The spent mushroom substrate (SS) and Microbacterium.sp.Q2 bacteria were used to remediate petroleum-contaminated soil and four different treatments which contained spent mushroom substrate immobilized microorganisms (SIM), spent mushroom substrate-free bacteria (SMSB), spent mushroom substrate alone (SMS) and no treatment (CK) were designed in this paper. The aim of this study is to investigate soil microbial number, enzyme activities and the degradation rate of total petroleum hydrocarbon (TPH), exploring an ideal reparative treatment. The results indicated that soil respiration intensity, microbial number and enzyme activities of SIM group were obviously higher than others and the removal rate of TPH was highest which was increased by 11.84%, 22.15% and 54.09% compared with other treatments. Furthermore it is showed that the dehydrogenase activity and microbial activity were significantly correlated with the degradation rate of TPH and immobilized microorganism had the synergistic mechanism of bioaugmentation and biostimulation for remediation of petroleum contaminated soil.