共代谢作用下芦苇根际细菌多样性与群落组成

以对羟基苯甲酸(PHA)、对香豆酸(PCA)、咖啡酸(CA)和阿魏酸(FA)为代表性酚类根系分泌物(PREs),以对叔丁基苯酚(PTBP)作为典型烷基酚、以常见湿地植物芦苇为受试植物,结合高通量测序和生物信息学分析,研究PREs-PTBP交互作用下芦苇根际细菌多样性与群落组成的变化规律.结果表明,所有PREs均能有效提...

Bacterial diversity and community composition in the Phragmites australis rhizosphere by cometabolism

p-hydroxybenzoic acid (PHA), p-coumaric acid (PCA), caffeic acid (CA), and ferulic acid (FA) were selected as the representative phenolic root exudates (PREs), p-tert-butylphenol (PTBP) was chosen as the typical alkylphenol, and common reed (Phragmites australis) was used as the test plant. High-throughput sequencing and bioinformatics analyses were applied to investigate the variation of bacterial diversity and community by PRE-PTBP interaction in Phragmites australis rhizosphere. PREs could effectively improve the rhizosphere bacterial activity and accelerate the organic carbon degradation. However, the PTBP biodegradation could be promoted by monophenol PREs (PHA, PCA and FA), but not by polyphenol PREs (CA). On the one hand, PREs could decide the dominant bacterial phyla as carbon source: Proteobacteria and Bacteroidetes with high carbon source utilization ability increased by 15.34% and 4.73% in +PRE treatments, respectively; whereas Actinobacteria and Firmicutes with better detoxification/tolerance capacity increased by 15.92% and 9.99% in -PRE treatments, respectively. On the other hand, the functional structure of PREs could determine the functional bacterial genus: monophenol PREs (PHA, PCA and FA) are conducive to enrich monophenol-degrading bacteria, like Pseudomonas, accounting for 1.45%~4.02%; polyphenol PREs (CA) are favored to increase the abundance of polyphenol-degrading bacteria, like Novosphingobium, accounting for 3.71%. Furthermore, simple structure of PREs is beneficial for the enrichment of ASVs. The closer the PRE structure are, the more similar the ASV species will be. The community composition of rhizosphere bacteria in different treatments is not a random phenomenon, but a directional adjustment of rhizosphere microbiome by exudates released from plants.