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Effects of permethrin exposure on antioxidant enzymes and protein status in Mediterranean clams Ruditapes decussatus |
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| Authors: | Badreddine Sellami Hela Louati Mohamed Dellali Patricia Aissa Ezzeddine Mahmoudi Ana Varela Coelho David Sheehan |
| Institution: | 1. Laboratory of Environment Biomonitoring, Coastal Ecology Unit, Faculty of Sciences of Bizerta, University of Carthage, 7021, Zarzouna, Tunisia 2. Mass Spectrometry Laboratory, Analytical Services Unit, Institute of Chemical and Biological Technology (ITQB), New University of Lisbon, Avenida República - Quinta do Marquês, 2784-505, Oeiras, Portugal 3. Environmental Research Institute and Department of Biochemistry, University College Cork, Western Gateway Building, Western Road, Cork, Ireland |
| Abstract: | The effects of permethrin (PER) on a panel of antoxidant enzymes; superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and indices of protein oxidation status (carbonylation and free thiols) were determined in digestive gland and gills of the clam Ruditapes decussatus. Animals were exposed to 100 ppb PER for 2 days. These enzyme activities increased significantly in digestive gland (p?<?0.05) after PER treatment and oxidative modification of proteins was detected in both gill and digestive gland extracts using redox proteomics. PER exposure significantly reduced the amount of protein free thiol groups in digestive gland rather than in gill, when compared to controls. Conversely, digestive gland showed significantly higher levels of carbonylated proteins than gill after PER exposure. Some proteins were successfully identified by mass spectrometry of tryptic peptides. Our data suggest that digestive gland of R. decussatus can be used as a model tissue for investigating environmental risk of PER contamination. |
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